RabMAb

Anti-Cathepsin B抗体[EPR4323] (ab125067)

概述

  • 产品名称Anti-Cathepsin B抗体[EPR4323]
    参阅全部 Cathepsin B 一抗
  • 描述
    兔单克隆抗体[EPR4323] to Cathepsin B
  • 经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Cathepsin B aa 1-200. The exact sequence is proprietary.

  • 阳性对照
    • Human kidney and papillary carcinoma tissues; HeLa cells; HT1080, A375, SW480 and A549 cell line lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab125067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
    • 序列相似性Belongs to the peptidase C1 family.
    • 细胞定位Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Amyloid precursor protein secretase antibody
      • APP secretase antibody
      • APPS antibody
      • CATB_HUMAN antibody
      • Cathepsin B heavy chain antibody
      • Cathepsin B1 antibody
      • CathepsinB antibody
      • CPSB antibody
      • CTSB antibody
      • cysteine protease antibody
      • OTTHUMP00000116009 antibody
      • OTTHUMP00000229510 antibody
      • OTTHUMP00000229511 antibody
      • OTTHUMP00000229512 antibody
      • OTTHUMP00000229514 antibody
      • OTTHUMP00000229515 antibody
      • OTTHUMP00000229516 antibody
      • Preprocathepsin B antibody
      see all

    Anti-Cathepsin B antibody [EPR4323] 图像

    • ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human kidney tissue by immunohistochemistry.
    • ab125067, at a 1/50 dilution, staining Cathepsin B (red) in HeLa cells by immunofluorescence. DNA is marked in blue.
    • All lanes : Anti-Cathepsin B antibody [EPR4323] (ab125067) at 1/1000 dilution

      Lane 1 : HT1080 cell lysate
      Lane 2 : A375 cell lysate
      Lane 3 : SW480 cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 38 kDa
    • ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human papillary carcinoma tissue by immunohistochemistry.

    Anti-Cathepsin B antibody [EPR4323] (ab125067)参考文献

    ab125067 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us and your interest in our products.
    Please find the ICC protocol used with the Anti-Cathepsin B antibody [EPR4323] (ab125067) attached to this email.
    The secondary antibody used in the staining was an Alexa Fluor 56...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"