Catalase Specific Activity Assay试剂盒(ab118184)

概述

  • 产品名称Catalase Specific Activity Assay试剂盒
    参阅全部 Catalase 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    1 8 7.1%
    批次间
    样品 n Mean SD CV%
    1 4 9.9%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture extracts, Tissue Extracts
  • 检测类型Enzyme activity
  • 范围
    1 µg/ml - 1000 µg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 产品概述

    ab118184, is used to determine the relative specific activity (activity and quantity) of catalase in a sample. The native enzyme is immunocaptured within the wells of the microplate; this removes all other enzymes. The assay buffer contains hydrogen peroxide which reacts with a substrate to make a luminescent product. Catalase functions rapidly to remove hydrogen peroxide from the solution and reduce the production of light. Therefore the light produced in each well is inversely proportional to the amount of catalase activity.

    After activity measurement the quantity of catalase is measured by adding to each well an anti-catalase detector antibody. After washing away unbound detector antibody, HRP-conjugated label specific for the detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of catalase bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.

  • 经测试应用适用于: Functional Studiesmore details
  • 平台Microplate

性能

  • 功能Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • 疾病相关Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • 序列相似性Belongs to the catalase family.
  • 翻译后修饰The N-terminus is blocked.
  • 细胞定位Peroxisome.
  • Information by UniProt
  • 别名
    • CAT
    • CATA_HUMAN
    • Catalase
    • EC 1.11.1.6
    • MGC138422
    • MGC138424
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab118184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

Catalase Specific Activity Assay Kit 图像

  • Example sample curves demonstrating the working range of the assay for a human and cultured cell line lysate (HepG2).
  • Example standard curve for the purified human catalase sample ab91026.
  • Example sample curves demonstrating the working range of the assay for a human and cultured cell line lysate (HepG2).
  • Example standard curve for the purified human catalase sample ab91026.
  • Example relative specific activity – comparing the activity and quantity of purified erythrocyte catalase (ab91026) and catalase from human hepg2 lysate. The HepG2 sample is determined to have a higher specific activity rate.

实验方案

Catalase Specific Activity Assay Kit (ab118184)参考文献

ab118184 has not yet been referenced specifically in any publications.

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