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Catalase (Bovine Liver)
Our Abpromise guarantee covers the use of ab15834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 60 kDa).|
ICC/IF image of ab15834 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15834, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).
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