Anti-Catalase抗体(HRP) (ab6573)

概述

  • 产品名称Anti-Catalase抗体(HRP)
    参阅全部 Catalase 一抗
  • 描述
    兔多克隆抗体to Catalase (HRP)
  • 偶联物HRP
  • 经测试应用适用于: Dot blot, Electron Microscopy, ELISA, IHC-Fr, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Cow
    预测可用于: all Mammals
  • 免疫原

    Catalase [Bovine Liver]

  • 常规说明

    Antibody reacts with the tetrameric hemeprotein catalase from all mammalian sources and likely most aerobic microorganisms. Catalase catalyzes the conversion of hydrogen peroxide to water and oxygen. This antibody is designed to detect the enzyme in most immunochemical methods. The antibody may facilitate the study of oxidative stress release in neuron transplantation.

性能

应用

Our Abpromise guarantee covers the use of ab6573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot Use at an assay dependent concentration.
Electron Microscopy Use at an assay dependent concentration.
ELISA 1/10000 - 1/40000.

This product has been assayed against 1.0 ug of Catalase (Bovine Liver) in a standard capture ELISA using ABTS (2,2prime-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.

IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 60 kDa).

靶标

  • 功能Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • 疾病相关Acatalasemia
  • 序列相似性Belongs to the catalase family.
  • 翻译后修饰The N-terminus is blocked.
  • 细胞定位Peroxisome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cas 1 antibody
    • CAT antibody
    • CATA_HUMAN antibody
    • Catalase antibody
    • Cs1 antibody
    see all

Anti-Catalase antibody (HRP) (ab6573)参考文献

This product has been referenced in:
  • Rajasekaran NS  et al. Human alpha B-crystallin mutation causes oxido-reductive stress and protein aggregation cardiomyopathy in mice. Cell 130:427-39 (2007). WB ; Mouse . Read more (PubMed: 17693254) »
  • Mobley JA & Brueggemeier RW Estrogen receptor-mediated regulation of oxidative stress and DNA damage in breast cancer. Carcinogenesis 25:3-9 (2004). Read more (PubMed: 14514655) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. We do not know the epitope that this antibody recognizes. The prediction is that the antibody reacts with all Mammals and most aerobic microorganisms due to sequence homology. I am unable to tell you if this antibody re...

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (10)
Sample Saccharomyces cerevisiae Cell lysate - whole cell (By4741)
Specification By4741
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Jun 17 2013

Thank you for yourr enquiry. Ab6572 and ab6573 are conjugation versions of ab1877. We do not have references for those antibodies, however we have references for ab1877: This product has been used in: Brandon NJ et al. Subcellular targeting of...

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The expected band size is around 56-59 kDa. If your customer has any more questions/concerns, just let us know.

Thank you for your enquiry. The expected band size is around 56-59 kDa.

This antibody has never been tested on FFPA sections, therefore we can't guarantee a result.

Try this antibody at 1:500 and adjust the dilution accordingly from the results obtained.

Judging from sequence analysis, it is quite possible that this antibody will detect catalase in Helicobacter pylori. However, because we have not actually tested this product on Helicobacter pylori, we can't guarantee that it will work.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"