概述

  • 产品名称Anti-Catalase抗体(Biotin)
    参阅全部 Catalase 一抗
  • 描述
    兔多克隆抗体to Catalase (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: Dot blot, ELISA, IHC-Fr, IP, WBmore details
  • 种属反应性
    与反应: Cow
    预测可用于: all Mammals
  • 免疫原

    Full length protein (Bovine Liver)

  • 常规说明

    Antibody reacts with the tetrameric hemeprotein catalase from all mammalian sources and likely most aerobic microorganisms. Catalase catalyzes the conversion of hydrogen peroxide to water and oxygen. This antibody is designed to detect the enzyme in most immunochemical methods. The antibody may facilitate the study of oxidative stress release in neuron transplantation.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
  • Primary antibody说明Antibody reacts with the tetrameric hemeprotein catalase from all mammalian sources and likely most aerobic microorganisms. Catalase catalyzes the conversion of hydrogen peroxide to water and oxygen. This antibody is designed to detect the enzyme in most immunochemical methods. The antibody may facilitate the study of oxidative stress release in neuron transplantation.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot Use at an assay dependent concentration.
ELISA 1/4000 - 1/20000.

This product has been assayed against 1.0 ug of Catalase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2prime-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.

IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

靶标

  • 功能Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • 疾病相关Acatalasemia
  • 序列相似性Belongs to the catalase family.
  • 翻译后修饰The N-terminus is blocked.
  • 细胞定位Peroxisome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cas 1 antibody
    • CAT antibody
    • CATA_HUMAN antibody
    • Catalase antibody
    • Cs1 antibody
    see all

Anti-Catalase antibody (Biotin) (ab6572)参考文献

This product has been referenced in:
  • Bergersen LH  et al. Immunogold detection of L-glutamate and D-serine in small synaptic-like microvesicles in adult hippocampal astrocytes. Cereb Cortex 22:1690-7 (2012). Read more (PubMed: 21914633) »
  • Duffy AM  et al. Generation of antioxidant adenovirus gene transfer vectors encoding CuZnSOD, MnSOD, and catalase. Methods Mol Biol 594:381-93 (2010). WB . Read more (PubMed: 20072932) »

See all 4 Publications for this product

Product Wall

Thank you for yourr enquiry. Ab6572 and ab6573 are conjugation versions of ab1877. We do not have references for those antibodies, however we have references for ab1877: This product has been used in: Brandon NJ et al. Subcellular targeting of...

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The catalase has MW of 250kDa. Sund et al. (1967) indicate 4 subunits of equal size (MW 60,000-65,000) in agreement with Shpitzberg cited by Kiseler et al. (1967). See also Furuta et al. (1974) and McPherson and Rich (1973). Fita and Rossmann (1985) an...

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Thank you for your enquiry. The molecular weights are listed below; we unfortunately do not have information regarding the isoelectric points. Ab16875 - Approximately 21 kDa Ab7333 - Approximately 28 kDa Ab16798 - Approximately 22 kDa Ab16800...

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The antibody is made and tested against Bovine catalase but other species have not been tested. The statement "likely to cross-react with 'most aerobic microorganisms'" is from sequence analysis. Other cross reactivities are unknown. Western Blot image...

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This is an interesting question - what is yourcustomer going to be doing exactly? Biotin is conjugated to the primary antibody, but I'm really not sure if there will be any interference.

The catalase source is bovine, and the antibody is polyclonal. Although it has not been tested on mouse tissue, the chances are that it will crossreact. We do not have any information regarding which is the better fixative for use with this antib...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"