Anti-Catalase抗体(ab98210)

概述

  • 产品名称Anti-Catalase抗体
    参阅全部 Catalase 一抗
  • 描述
    兔多克隆抗体to Catalase
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 467-516 (LKDAQIFIQK KAVKNFTEVH PDYGSHIQAL LDKYNAEKPK NAIHTFVQSG) of Human Catalase (NP_001743).

  • 阳性对照
    • Human fetal heart lysate

性能

应用

Our Abpromise guarantee covers the use of ab98210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.

靶标

Anti-Catalase antibody 图像

  • Anti-Catalase antibody (ab98210) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Gel concentration: 12%
  • ab98210 staining Catalase in murine J774 macrophages by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in 5% paraformaldehyde and permeabilized using ice cold acetone, blocked with 30% human serum, 0.1% BSA, 1% fish gelatin, 5 mM EDTA, and 0.2% Tween 20 in PBS for 30 minutes at 4°C. Samples were then incubated with ab98210 at a 1/100 dilution for 1 hour at 4°C. The secondary used was an Alexa Fluor 488 conjugated antibody used at a 1/1000 dilution. Counterstained with DAPI.

Anti-Catalase antibody (ab98210)参考文献

ab98210 has not yet been referenced specifically in any publications.

Product Wall

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Thank you for sending the image which is very self explanatory for multiple bands. Please ignore my suggestions for multiple bands as these were more general rather than related to this case. The antibody (ab98210) I recommended was the second most...

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Thank you for your email. I am sorry to hear that the results did not improve. The multiple band problem could be due to following reasons: - High protein conc. of lysates per well - High conc. of primary and secondary ab - Cell confluence or ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this ha...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"