概述

  • 产品名称Anti-Catalase抗体
    参阅全部 Catalase 一抗
  • 描述
    山羊多克隆抗体to Catalase
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human, Pig
    预测可用于: Mouse, Guinea pig, Cow, Dog, Marmoset (common), Orangutan
  • 免疫原

    Synthetic peptide:

    C-DKYNAEKPKNAIHT

    , corresponding to internal sequence amino acids 498-511 of Human Catalase

  • 阳性对照
    • Human Kidney and Liver lysates.

性能

应用

Our Abpromise guarantee covers the use of ab50434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IP Use at an assay dependent dilution. PubMed: 20923778
IHC-P Use a concentration of 2 - 4 µg/ml.

靶标

Anti-Catalase antibody 图像

  • Anti-Catalase antibody (ab50434) at 1 µg/ml + Human Kidney lysate (35µg protein in RIPA buffer).

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
  • ab50434 at 2µg/ml staining Human liver tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by steam in citrate buffer pH 6.0. The AP staining procedure was used.

Anti-Catalase antibody (ab50434)参考文献

This product has been referenced in:
  • Habib LK  et al. Inhibitors of Catalase-Amyloid Interactions Protect Cells from {beta}-Amyloid-Induced Oxidative Stress and Toxicity. J Biol Chem 285:38933-43 (2010). IP ; Human . Read more (PubMed: 20923778) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Pig Tissue lysate - whole (coronary arteries and arterioles)
Loading amount 20 µg
Specification coronary arteries and arterioles
Gel Running Conditions Non-reduced Non-Denaturing (Native) (12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5µg/mL · Temperature: 37°C
Username

Dr. Wei Xie

Verified customer

提交于 Jul 13 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"