Anti-Catalase抗体[1A1] (ab16771)

概述

  • 产品名称Anti-Catalase抗体[1A1]
    参阅全部 Catalase 一抗
  • 描述
    小鼠单克隆抗体[1A1] to Catalase
  • 经测试应用适用于: IP, ELISA, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein (Human).

  • 阳性对照
    • HeLa whole cell lysate. IF/ICC: HepG2 cell line.

性能

应用

Our Abpromise guarantee covers the use of ab16771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 19659692
Flow Cyt Use 1µg for 106 cells. (Also see PMID: 19659692)



ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • 疾病相关Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • 序列相似性Belongs to the catalase family.
  • 翻译后修饰The N-terminus is blocked.
  • 细胞定位Peroxisome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cas1 antibody
    • CAT antibody
    • CATA_HUMAN antibody
    • Catalase antibody
    • Cs1 antibody
    • MGC138422 antibody
    • MGC138424 antibody
    see all

Anti-Catalase antibody [1A1] 图像

  • ICC/IF image of ab16771 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16771, 1/50 dilution) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunoprecipitation analysis of HeLa cell lysates with ab16771.

    Lane  1: Input. Lane 2: Precipitates.

  • Overlay histogram showing HeLa cells stained with ab16771 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16771, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Catalase antibody [1A1] (ab16771)参考文献

This product has been referenced in:
  • Zhang J  et al. A tuberous sclerosis complex signalling node at the peroxisome regulates mTORC1 and autophagy in response to ROS. Nat Cell Biol 15:1186-96 (2013). WB, IF . Read more (PubMed: 23955302) »
  • Baarine M  et al. Peroxisomal and mitochondrial status of two murine oligodendrocytic cell lines (158N, 158JP): potential models for the study of peroxisomal disorders associated with dysmyelination processes. J Neurochem 111:119-31 (2009). ICC/IF, Flow Cyt ; Mouse . Read more (PubMed: 19659692) »

See all 2 Publications for this product

Product Wall


As shown on the product datasheet, http://www.abcam.com/ab16771, upon delivery we recommend toaliquot and store this product at -20°C. At -20ºC an antibody can be kept for 5 years. Avoid freeze / thaw cycles.

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver, Tonsil, Lung and pellets from cell lines:HE)
Specification Liver, Tonsil, Lung and pellets from cell lines:HE
Fixative Formaldehyde
Antigen retrieval step Other
Permeabilization No
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Abcam user community

Verified customer

提交于 Sep 28 2010

Thank you for getting back to me. I am sorry for the delay, I was waiting for a response from the source of this antiserum. I have been able to obtain a recommended protocol for IP using this antiserum. However, this is still yet to be tested for t...

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Thank you for getting back to me. I am sorry for any confusion. ab16771 IS capable of immunoprecipitation. This was confirmed by performing immunoprecipitation followed by western blotting. However, immunoblotting detection was performed under DENA...

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Thank you for your enquiry. ab16771 has been shown to immunoprecipitate Catalase and to cross react against rat. Furthermore ab1877 and ab16772 have also been shown to immunoprecipitate Catalase in rat. Unfortunately all three antisera are yet to d...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"