使用敲除细胞株进行验证

Anti-Catalase抗体[12C2DB9] - Peroxisome Marker (ab110292)

概述

  • 产品名称
    Anti-Catalase抗体[12C2DB9] - Peroxisome Marker
    参阅全部 Catalase 一抗
  • 描述
    小鼠单克隆抗体[12C2DB9] to Catalase - Peroxisome Marker
  • 宿主
    Mouse
  • 经测试应用
    适用于: ICC, IP, Flow Cyt, In-Cell ELISAmore details
  • 种属反应性
    与反应: Rat, Cow, Human
  • 免疫原

    Rat liver mitochondria

  • 阳性对照
    • HeLa cells, HepG2 cells, HL60 cells, Cow heart, Rat liver Flow cyt: HAP1-WT cells.
  • 常规说明

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

性能

应用

Our Abpromise guarantee covers the use of ab110292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration.
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

In-Cell ELISA Use a concentration of 4 µg/ml.

靶标

  • 功能
    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • 疾病相关
    Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • 序列相似性
    Belongs to the catalase family.
  • 翻译后修饰
    The N-terminus is blocked.
  • 细胞定位
    Peroxisome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cas1 antibody
    • CAT antibody
    • CATA_HUMAN antibody
    • Catalase antibody
    • Cs1 antibody
    • MGC138422 antibody
    • MGC138424 antibody
    see all

图片

  • Overlay histogram showing HAP1 wildtype (green line) and HAP1-CAT knockout cells (red line) stained with ab110292. The cells were fixed with 4% formaldehyde (10 min)  and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab110292, 1µg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) presorbed (ab150117) at 1/2000 dilution for 30 min at 22°C.

    A mouse IgG1 isotype control antibody  (ab170190) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-CAT  knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

  • Immunocytochemical staining of Human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the ab110292 (5 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody, Alexa Fluor® 488 goat anti-mouse IgG (green), was used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). The target protein locates mainly in peroxisomes.
  • Immunoprecipitation of Catalase using ab110292 from:
    Lane 1: HepG2 cells
    Lane 2: Cow heart sample
    Lane 3: Rat liver sample

    Predicted band size is 60 kDa.
  • Flow cytometric analysis of HL-60 cells using ab110292 at 1 µg/mL, (blue) or an isotype control (red).

文献

ab110292 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you very much for contacting us.

Unfortunately, the dissociation constant KD is not routinely determined and we therefore cannot provide this information on our antibodies against bovine catalase.

Having said this, I am happ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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