Catalase Activity Assay试剂盒(Colorimetric/Fluorometric) (ab83464)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell Lysate, Plasma, Serum, Tissue Lysate, Urine
概述
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产品名称
Catalase Activity Assay试剂盒(Colorimetric/Fluorometric)
参阅全部 Catalase 试剂盒 -
检测方法
Colorimetric/Fluorometric -
样品类型
Urine, Serum, Plasma, Cell Lysate, Tissue Lysate -
检测类型
Enzyme activity (quantitative) -
检测时间
0h 40m -
种属反应性
与反应: Mammals -
产品概述
Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids.
In the catalase activity assay protocol, the catalase present in the sample reacts with hydrogen peroxide (H2O2) to produce water and oxygen. The unconverted H2O2 reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm or fluorometrically at Ex/Em = 535/587 nm. Therefore, the catalase activity present in the sample is reversely proportional to the signal obtained. The kit can detect as little as 1 µU of catalase activity.
Catalase activity assay protocol summary:
- add samples and standards to wells
- add stop solution into sample control wells and incubate at 25ºC for 5 min
- add H2O2 solution into wells and incubate for 30 min at 25ºC
- add stop solution
- add developer mix and incubate for 10 min at 25ºC
- analyze with microplate reader -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K773 Catalase Activity Colorimetric/Fluorometric Assay Kit. K773-100 is the same size as the 100 test size of ab83464.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests 2000 tests 100 tests Assay Buffer VIII 1 x 25ml 20 x 25ml 1 x 25ml Catalase Positive Control 1 x 20µl 20 x 20µl 1 x 2µl Developer Solution V 1 vial 20 vials 1 vial Hydrogen Peroxide Solution II 1 x 25µl 20 x 25µl 1 x 25µl OxiRed Probe 1 x 200µl 20 x 200µl 1 x 200µl Stop Solution III 1 x 1ml 20 x 1ml 1 x 1ml -
研究领域
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功能
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. -
疾病相关
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions. -
序列相似性
Belongs to the catalase family. -
翻译后修饰
The N-terminus is blocked. -
细胞定位
Peroxisome. - Information by UniProt
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别名
- Cas1
- CAT
- CATA_HUMAN
see all
数据表及文件
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SDS download
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Datasheet download
文献 (84)
ab83464 被引用在 84 文献中.
- Meng J & Xu C MicroRNA-495-3p diminishes doxorubicin-induced cardiotoxicity through activating AKT. J Cell Mol Med 26:2076-2088 (2022). PubMed: 35152537
- Abbas AO et al. Citrullus colocynthis Seed Ameliorates Layer Performance and Immune Response under Acute Oxidative Stress Induced by Paraquat Injection. Animals (Basel) 12:N/A (2022). PubMed: 35454193
- Zhang D et al. Hydrogen Peroxide Scavenging Restores N-Type Calcium Channels in Cardiac Vagal Postganglionic Neurons and Mitigates Myocardial Infarction-Evoked Ventricular Arrhythmias in Type 2 Diabetes Mellitus. Front Cardiovasc Med 9:871852 (2022). PubMed: 35548411
- Younis NS ß-Caryophyllene Ameliorates Cyclophosphamide Induced Cardiac Injury: The Association of TLR4/NF?B and Nrf2/HO1/NQO1 Pathways. J Cardiovasc Dev Dis 9:N/A (2022). PubMed: 35621844
- Balta I et al. The Antioxidant Effect of Natural Antimicrobials in Shrimp Primary Intestinal Cells Infected with Nematopsis messor. Antioxidants (Basel) 11:N/A (2022). PubMed: 35624838