概述

  • 产品名称Anti-CAT1抗体
    参阅全部 CAT1 一抗
  • 描述
    兔多克隆抗体to CAT1
  • 经测试应用适用于: IHC-FoFr, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human CAT1 (gene: SLC7A1).

    (Peptide available as ab37587.)

  • 阳性对照
    • This antibody gave a positive signal in the following human whole cell lysates: Jurkat, MCF-7 and HepG2.

性能

应用

Our Abpromise guarantee covers the use of ab37588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr 1/1000.
WB 1/250. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).

Abcam recommends using milk as the blocking agent.

靶标

  • 功能High-affinity, low capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine) in non-hepatic tissues. May also function as an ecotropic retroviral leukemia receptor.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amino acid transporter cationic 1 antibody
    • ATRC1 antibody
    • CAT-1 antibody
    • CaT1 antibody
    • CTR1_HUMAN antibody
    • Ecotropic retroviral leukemia receptor homolog antibody
    • Ecotropic retroviral receptor antibody
    • Ecotropic retrovirus receptor homolog antibody
    • ERR antibody
    • HCAT1 antibody
    • High affinity cationic amino acid transporter 1 antibody
    • High-affinity cationic amino acid transporter-1 antibody
    • REC1L antibody
    • Slc7a1 antibody
    • Solute carrier family 7 (cationic amino acid transporter, y+ system) member 1 antibody
    • Solute carrier family 7 member 1 antibody
    • System Y+ basic amino acid transporter antibody
    see all

Anti-CAT1 antibody 图像

  • All lanes : Anti-CAT1 antibody (ab37588) at 1 µg/ml

    Lane 1 : Jurkat (Human) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate
    Lane 4 : LNCaP (Human prostate carcinoma) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Additional bands at : 160 kDa,76 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab37588 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  • All lanes : Anti-CAT1 antibody (ab37588) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
  • All lanes : Anti-CAT1 antibody (ab37588) at 1 µg/ml (3% Milk)

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Additional bands at : 135 kDa,155 kDa,76 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab37588 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  • IHC-FoFr image of CAT1 staining on Mouse Spinal Cord sections using ab37588 (1:1000). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the spinal cord were cryoprotected in sucrose 30% overnight. Spinal cords were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

    See Abreview

Anti-CAT1 antibody (ab37588)参考文献

This product has been referenced in:
  • Wang X  et al. Functional role of arginine during the peri-implantation period of pregnancy. II. Consequences of loss of function of nitric oxide synthase NOS3 mRNA in ovine conceptus trophectoderm. Biol Reprod 91:59 (2014). Read more (PubMed: 25061098) »
  • Battisti S  et al. Nutritional stress and arginine auxotrophy confer high sensitivity to chloroquine toxicity in mesothelioma cells. Am J Respir Cell Mol Biol 46:498-506 (2012). Read more (PubMed: 22074703) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us.

The anti-SLC7A1 antibody ab37588 has been tested for reactivity with PFA-fixed, frozen mouse spinal chord by IHC. An image of the stain and a brief description of the protocol are available on the online datashee...

Read More
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (T cell)
Specification T cell
Permeabilization No
Gating Strategy living cells
Username

Ms. Beck

Verified customer

提交于 Sep 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Spinal cord)
Specification Spinal cord
Username

Dr. Sophie Pezet

Verified customer

提交于 May 17 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"