概述

  • 产品名称Anti-Caspr抗体
    参阅全部 Caspr 一抗
  • 描述
    兔多克隆抗体to Caspr
  • 经测试应用适用于: IHC-P, WB, ICC/IF, IP, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1350 to the C-terminus of Mouse Caspr.

    (Peptide available as ab34150.)

  • 阳性对照
    • Rat brain whole cell lysate and PC12 cytoplasmic lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab34151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB 1/250. Detects a band of approximately 180 kDa (predicted molecular weight: 156 kDa).Can be blocked with Mouse Caspr peptide (ab34150).
ICC/IF Use a concentration of 1 µg/ml.
IP Use at an assay dependent concentration.
IHC-FoFr 1/3000.

靶标

  • 功能Seems to play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the paranodal region of the axo-glial junction. In association with contactin may have a role in the signaling between axons and myelinating glial cells.
  • 组织特异性Predominantly expressed in brain. Weak expression detected in ovary, pancreas, colon, lung, heart, intestine and testis.
  • 序列相似性Belongs to the neurexin family.
    Contains 2 EGF-like domains.
    Contains 1 F5/8 type C domain.
    Contains 1 fibrinogen C-terminal domain.
    Contains 4 laminin G-like domains.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Caspr antibody
    • Caspr1 antibody
    • CNTNAP antibody
    • Cntnap1 antibody
    • CNTP1_HUMAN antibody
    • Contactin associated protein 1 antibody
    • Contactin-associated protein 1 antibody
    • MHDNIV antibody
    • NCP1 antibody
    • Neurexin 4 antibody
    • Neurexin IV antibody
    • Neurexin-4 antibody
    • Nrxn4 antibody
    • p190 antibody
    • Paranodin antibody
    see all

Anti-Caspr antibody 图像

  • Anti-Caspr antibody (ab34151) at 1/250 dilution + Brain (Rat) Whole Cell Lysate - normal tissue at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 156 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.
    Caspr contains a number of potential glycosylation sites so it is thought that this is the reason it runs at 180kDa.
  • Caspr was immunoprecipitated using 0.5mg Rat Brain whole tissue lysate, 5µg of Rabbit polyclonal to Caspr and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Rat Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab34151.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 180kDa: Caspr.
  • ICC/IF image of ab34151 stained SHSY5Y cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab34151, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • IHC-FoFR image of ab34151 stained sections of mouse brain (30 um). The tissues were from perfused fixed animals perfused with 4% PFA and postfixed 2h in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.

    See Abreview

  • ab34151 staining Caspr in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde. Samples were blocked with 20% serum for 1 hour at 20°C followed by incubation with the primary antibody at a 1/300 dilution for 12 hours at 20°C. An HRP-conjugated goat anti-rabbit polyclonal was used as the secondary antibody at a 1/200 dilution.

    See Abreview

Anti-Caspr antibody (ab34151)参考文献

This product has been referenced in:
  • Brügger V  et al. HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins. PLoS Biol 13:e1002258 (2015). Read more (PubMed: 26406915) »
  • McLean NA  et al. Delayed nerve stimulation promotes axon-protective neurofilament phosphorylation, accelerates immune cell clearance and enhances remyelination in vivo in focally demyelinated nerves. PLoS One 9:e110174 (2014). IHC-Fr ; Rat . Read more (PubMed: 25310564) »

See all 12 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C
Antigen retrieval step None
Sample Mouse Tissue sections (brain)
Specification brain
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 21 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Dorsal root ganglion)
Specification Dorsal root ganglion
Username

Dr. Sophie Pezet

Verified customer

提交于 Aug 03 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"