Caspase 9抑制剂Drug Detection试剂盒(ab102497)


  • 产品名称
    Caspase 9抑制剂Drug Detection试剂盒
    参阅全部 Caspase 9 试剂盒
  • 检测方法
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型
  • 检测时间
    1h 30m
  • 产品概述

    Abcam's Caspase 9 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate LEHD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 9 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 9 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.

  • 经测试应用
    适用于: Functional Studiesmore details
  • 平台


  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 100 tests
    2X Reaction Buffer 1 x 10ml
    Active Caspase-9 1 x 100 units
    Caspase Inhibitor, Z-VAD-FMK (2 mM) 1 x 10µl
    Caspase Substrate LEHD-AFC (1 mM) 1 x 0.5ml
    DTT (1 M) 1 x 100µl
  • 研究领域
  • 相关性
    Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. It is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 is approximately 47 kD. It is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. It becomes activated when recruited to the Apaf1/cytochrome c complex.
  • 别名
    • APAF3
    • CASP9
    • Caspase 9
    • ICE LAP6
    • MCH6
    see all



Our Abpromise guarantee covers the use of ab102497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.


  • Titration of the caspase 9 inhibitor Z-LEHD-FMK (ab142026) (duplicates; +/- SD).

  • Titration of the caspase inhibitors Z-VAD(OMe)-FMK (ab120487) and Q-LEHD-Oph (ab142039) (duplicates; +/- SD).

  • Titration of caspase inhibitor Z-VAD-FMK (ab102497) (duplicates; +/- SD).

  • Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using LEHD-AFC as caspase 9 substrate and following Caspase 9 Inhibitor Drug Detection Kit (ab102493) protocol. Lanes:

    1.- Background Control: no Caspase 9, no z-VMAD-FMK

    2.- Positive Inhibition Control: no Caspase 9, +z-VAD-FMK

    3.- Positive Control: + Caspase 9, no z-VAD-FMK

    4.- Caspase 9 + 2.5µM z-VAD-FMK

    5.- Caspase 9 + 10µM z-VAD-FMK

    6.- Caspase 9 + 40µM z-VAD-FMK



ab102497 has not yet been referenced specifically in any publications.


Thank you very much for your email and for your patience while I've been in touch with the lab regarding your enquiry.

My contact at the lab has informed me that the background control is the fluorescence emitted by the substrate and the reac...

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Thank you for your inquiry.

1) We have not looked at decreasing volumes for 384-well plates. You may be
able to use these, but it will depend on the amount of caspase in the samples
and hence signal strength can be impacted.

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