Caspase-9抑制剂Assay试剂盒(ab102497)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Adherent cells, Suspension cells
概述
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产品名称
Caspase-9抑制剂Assay试剂盒
参阅全部 Caspase-9 试剂盒 -
检测方法
Fluorescent -
样品类型
Adherent cells, Suspension cells -
检测类型
Quantitative -
检测时间
1h 30m -
产品概述
Abcam's Caspase 9 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate LEHD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 9 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 9 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
Visit our FAQs page for tips and troubleshooting. -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K159 Caspase-9 Inhibitor Drug Screening Kit (Fluorometric). K159-100 is the same size as the 100 test size of ab102497.
Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests 2X Reaction Buffer 1 x 10ml Active Caspase-9 1 x 100 units Caspase Inhibitor, Z-VAD-FMK (2 mM) 1 x 10µl Caspase Substrate LEHD-AFC (1 mM) 1 x 0.5ml DTT (1 M) 1 x 100µl -
研究领域
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相关性
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. It is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 is approximately 47 kD. It is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. It becomes activated when recruited to the Apaf1/cytochrome c complex. -
别名
- APAF3
- CASP9
- Caspase 9
see all
相关产品
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Related Products
图片
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Titration of the caspase 9 inhibitor Z-LEHD-FMK (ab142026) (duplicates; +/- SD).
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Titration of caspase inhibitor Z-VAD-FMK (ab102497) (duplicates; +/- SD).
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Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using LEHD-AFC as caspase 9 substrate and following Caspase 9 Inhibitor Drug Detection Kit (ab102493) protocol. Lanes:
1.- Background Control: no Caspase 9, no z-VMAD-FMK
2.- Positive Inhibition Control: no Caspase 9, +z-VAD-FMK
3.- Positive Control: + Caspase 9, no z-VAD-FMK
4.- Caspase 9 + 2.5µM z-VAD-FMK
5.- Caspase 9 + 10µM z-VAD-FMK
6.- Caspase 9 + 40µM z-VAD-FMK
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab102497 尚未被引用在任何文献中。