Caspase 9 Assay试剂盒(Fluorometric) (ab65607)


  • 产品名称
    Caspase 9 Assay试剂盒(Fluorometric)
    参阅全部 Caspase 9 试剂盒
  • 样品类型
    Tissue Extracts, Cell Lysate
  • 检测类型
    Enzyme activity
  • 检测时间
    2h 00m
  • 产品概述

    Abcam's Caspase 9 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.
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  • 说明

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • 经测试应用
    适用于: Functional Studiesmore details


  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 100 tests
    2X Reaction Buffer 4 x 2ml
    Cell Lysis Buffer 1 x 100ml
    DTT 1 x 400µl
    LEHD-AFC (1mM) 1 x 500µl
  • 研究领域
  • 相关性
    Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. It is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 is approximately 47 kD. It is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. It becomes activated when recruited to the Apaf1/cytochrome c complex.
  • 别名
    • APAF3
    • CASP9
    • Caspase 9
    • ICE LAP6
    • MCH6
    see all



Our Abpromise guarantee covers the use of ab65607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.


  • Caspase 9 activity in Jurkat cells following 24 hour exposure to 50 ng x mL-1 anti-Fas Ab (MBL), 2 uM or 10 uM Camptothecin (CPT; ab120115) with or without 20 nM caspase inhibitor Z-VAD(OMe)-FMK (Zvad; ab120487). Sample input 5-25 uL, background signal subtracted, , duplicates; +/- SD.



This product has been referenced in:
  • Zou ZZ  et al. Synergistic induction of apoptosis by salinomycin and gefitinib through lysosomal and mitochondrial dependent pathway overcomes gefitinib resistance in colorectal cancer. Oncotarget 8:22414-22432 (2017). Functional Studies ; Human . Read more (PubMed: 26461472) »
  • Buvall L  et al. Orellanine specifically targets renal clear cell carcinoma. Oncotarget 8:91085-91098 (2017). Functional Studies . Read more (PubMed: 29207627) »

See all 3 Publications for this product


Thank you for calling Abcam earlier today.

I talked to the lab and they confirmed that you will have to read the signal with the wavelengths for excitation set at 400 nm and emission set at 505 nm. Youonly take one reading.

Hope this ...

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