Caspase 9 Assay试剂盒(Fluorometric) (ab65607)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Tissue Extracts
概述
-
产品名称
Caspase 9 Assay试剂盒(Fluorometric)
参阅全部 Caspase-9 试剂盒 -
检测方法
Fluorescent -
样品类型
Tissue Extracts, Cell Lysate -
检测类型
Enzyme activity -
检测时间
2h 00m -
产品概述
Abcam's Caspase 9 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.
Visit our FAQs page for tips and troubleshooting. -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K118 Caspase-9 Fluorometric Assay Kit. K118-100 is the same size as the 100 test size of ab65607.
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
Other caspase and apoptosis assays
Review the full set of caspase assays, or the apoptosis assay and apoptosis marker guide.
-
平台
Microplate reader
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests 2X Reaction Buffer 4 x 2ml Cell Lysis Buffer 1 x 100ml DTT 1 x 400µl LEHD-AFC (1mM) 1 x 500µl -
研究领域
-
相关性
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. It is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 is approximately 47 kD. It is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. It becomes activated when recruited to the Apaf1/cytochrome c complex. -
别名
- APAF3
- CASP9
- Caspase 9
see all
相关产品
-
Related Products
图片
数据表及文件
-
SDS download
-
Datasheet download
文献 (20)
ab65607 被引用在 20 文献中.
- Ding Z et al. Comparison of oxidative stress-mitochondria-mediated tenderization in two different bovine muscles during aging. Food Chem (Oxf) 5:100131 (2022). PubMed: 36060473
- Lu Y et al. The unfolded protein response to PI*Z alpha-1 antitrypsin in human hepatocellular and murine models. Hepatol Commun 6:2354-2367 (2022). PubMed: 35621045
- Doke T et al. Genome-wide association studies identify the role of caspase-9 in kidney disease. Sci Adv 7:eabi8051 (2021). PubMed: 34739325
- Ding D et al. Some Ototoxic Drugs Destroy Cochlear Support Cells Before Damaging Sensory Hair Cells. Neurotox Res 37:743-752 (2020). PubMed: 31997155
- Berthenet K et al. Failed Apoptosis Enhances Melanoma Cancer Cell Aggressiveness. Cell Rep 31:107731 (2020). PubMed: 32521256