Caspase 9 Assay试剂盒(Colorimetric) (ab65608)

概述

  • 产品名称
    Caspase 9 Assay试剂盒(Colorimetric)
    参阅全部 Caspase 9 试剂盒
  • 样品类型
    Tissue Extracts, Cell Lysate
  • 检测类型
    Enzyme activity
  • 检测时间
    2h 00m
  • 产品概述

    Abcam's Caspase 9 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate LEHD-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
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  • 说明

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • 经测试应用
    适用于: Functional Studiesmore details

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 100 tests
    2X Reaction Buffer 4 x 2ml
    Cell Lysis Buffer 1 x 100ml
    Dilution Buffer 1 x 100ml
    DTT 1 x 400µl
    LEHD-pNA (4mM) 1 x 500µl
  • 研究领域
  • 相关性
    Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. It is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 is approximately 47 kD. It is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. It becomes activated when recruited to the Apaf1/cytochrome c complex.
  • 别名
    • APAF3
    • CASP9
    • Caspase 9
    • ICE LAP6
    • MCH6
    see all

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应用

Our Abpromise guarantee covers the use of ab65608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

实验方案

文献

This product has been referenced in:
  • Zhu X  et al. Ziyuglycoside I Inhibits the Proliferation of MDA-MB-231 Breast Carcinoma Cells through Inducing p53-Mediated G2/M Cell Cycle Arrest and Intrinsic/Extrinsic Apoptosis. Int J Mol Sci 17:N/A (2016). Functional Studies . Read more (PubMed: 27879682) »
  • El Zawily AM  et al. The intrinsically kinase-inactive EPHB6 receptor predisposes cancer cells to DR5-induced apoptosis by promoting mitochondrial fragmentation. Oncotarget 7:77865-77877 (2016). Functional Studies . Read more (PubMed: 27788485) »

See all 5 Publications for this product

客户评价及客户问答

Thank you for your enquiry. Indeed, the kits does contain a cell lysis buffer that can be used for tissue homogenising. However, if you have prepared your samples already in your own lysis buffer, you may also dilute 100-200 ug cytosolic protein extrac...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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