Abcam's Caspase 9 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate LEHD-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
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Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
组件 | 100 tests |
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2X Reaction Buffer | 4 x 2ml |
Cell Lysis Buffer | 1 x 100ml |
Dilution Buffer | 1 x 100ml |
DTT | 1 x 400µl |
LEHD-pNA (4mM) | 1 x 500µl |
Our Abpromise guarantee covers the use of ab65608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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Functional Studies | Use at an assay dependent dilution. |
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"