Caspase 9 Assay试剂盒(Colorimetric) (ab65608)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Tissue Extracts
概述
-
产品名称
Caspase 9 Assay试剂盒(Colorimetric)
参阅全部 Caspase-9 试剂盒 -
检测方法
Colorimetric -
样品类型
Tissue Extracts, Cell Lysate -
检测类型
Enzyme activity -
检测时间
2h 00m -
种属反应性
与反应: Mammals -
产品概述
Caspase 9 Assay Kit (Colorimetric) ab65608 provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD.
The caspase 9 assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate LEHD-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
-
说明
This product is manufactured by BioVision, an Abcam company and was previously called K119 Caspase-9 Colorimetric Assay Kit. K119-100 is the same size as the 100 test size of ab65608.
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
Other caspase and apoptosis assays
Review the full set of caspase assays, or the apoptosis assay and apoptosis marker guide.
-
平台
Microplate reader
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests 2X Reaction Buffer I 4 x 2ml Dilution Buffer II 1 x 100ml DTT I 1 x 400µl LEHD-pNA 1 x 500µl Lysis Buffer IV 1 x 100ml -
研究领域
-
相关性
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. It is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 is approximately 47 kD. It is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. It becomes activated when recruited to the Apaf1/cytochrome c complex. -
别名
- APAF3
- CASP9
- Caspase 9
see all
相关产品
-
Related Products
图片
数据表及文件
-
SDS download
-
Datasheet download
文献 (64)
ab65608 被引用在 64 文献中.
- Gan K et al. Positive feedback loop of lncRNA FAM201A/miR-146a-5p/POU2F1 regulates IL-1ß-induced chondrocyte injury in vitro. Mol Med Rep 25:N/A (2022). PubMed: 34796909
- Kirman DC et al. Cell surface nucleolin is a novel ADAMTS5 receptor mediating endothelial cell apoptosis. Cell Death Dis 13:172 (2022). PubMed: 35197459
- Lai MC et al. The Citrus Flavonoid Hesperetin Encounters Diabetes-Mediated Alzheimer-Type Neuropathologic Changes through Relieving Advanced Glycation End-Products Inducing Endoplasmic Reticulum Stress. Nutrients 14:N/A (2022). PubMed: 35215394
- Bashir R et al. The modulation of PI3K/Akt pathway by 3β hydroxylup-12-en-28-oic acid isolated from Thymus linearis induces cell death in HCT-116 cells. Chem Biol Drug Des 99:162-178 (2022). PubMed: 34558199
- Mohamed ME et al. The Protective Effect of Anethole against Renal Ischemia/Reperfusion: The Role of the TLR2,4/MYD88/NF?B Pathway. Antioxidants (Basel) 11:N/A (2022). PubMed: 35326185