概述

  • 产品名称
    Anti-Caspase-9抗体[E84]
    参阅全部 Caspase-9 一抗
  • 描述
    兔单克隆抗体[E84] to Caspase-9
  • 经测试应用
    适用于: WB, Flow Cyt, IHC-Pmore details
    不适用于: ICC or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues near N-terminus of human Caspase-9

  • 阳性对照
    • Jurkat cell lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab32068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/25.
  • 应用说明
    Is unsuitable for ICC or IP.
  • 靶标

    • 功能
      Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
      Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
    • 组织特异性
      Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
    • 序列相似性
      Belongs to the peptidase C14A family.
      Contains 1 CARD domain.
    • 发展阶段
      Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
    • 翻译后修饰
      Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
    • Information by UniProt
    • 数据库链接
    • 别名
      • APAF-3 antibody
      • APAF3 antibody
      • Apoptosis related cysteine peptidase antibody
      • Apoptotic protease Mch-6 antibody
      • Apoptotic protease-activating factor 3 antibody
      • CASP-9 antibody
      • CASP9 antibody
      • CASP9_HUMAN antibody
      • Caspase 9 apoptosis related cysteine peptidase antibody
      • Caspase 9 Dominant Negative antibody
      • Caspase 9c antibody
      • Caspase-9 antibody
      • Caspase-9 subunit p10 antibody
      • ICE LAP6 antibody
      • ICE like apoptotic protease 6 antibody
      • ICE-LAP6 antibody
      • ICE-like apoptotic protease 6 antibody
      • MCH6 antibody
      • PPP1R56 antibody
      • protein phosphatase 1, regulatory subunit 56 antibody
      • RNCASP9 antibody
      see all

    Anti-Caspase-9 antibody [E84] 图像



    • Predicted band size : 46 kDa

      Lanes:
      Lane 1: Wild type HAP1 whole cell lysate (40 µg).
      Lane 2: CASP9 knockout  HAP1 whole cell lysate (40µg).
      Lane 3: HeLa  whole cell lysate (40 µg).

      Lanes 1 - 3 Merged signal (red and green). Green - ab32068 observed at 45 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32068 was shown to recognize CASP9 when CASP9 knockout samples were used, along with additional cross-reactive bands. Wild-type and CASP9 knockout samples were subjected to SDS-PAGE. Ab32068 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Caspase-9 antibody [E84] (ab32068) at 1/1000 dilution

      Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HeLa treated with staurosporine 1uM for 4 hours whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 46 kDa
      Additional bands at : 35 kDa,37 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes

      Blocking/dilution buffer: 5% NFDM/TBST.

    • Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-Pro-Caspase-9 RabMAb (ab32068).
    • Anti-Caspase-9 antibody [E84] (ab32068) at 1/2000 dilution + Jurkat cell lysate at 10 µg

      Predicted band size : 46 kDa
      Observed band size : 46 kDa

    Anti-Caspase-9 antibody [E84] (ab32068)参考文献

    This product has been referenced in:
    • Wang C  et al. JD enhances the anti-tumour effects of low-dose paclitaxel on gastric cancer MKN45 cells both in vitro and in vivo. Cancer Chemother Pharmacol 78:971-982 (2016). Read more (PubMed: 27620208) »
    • Catuogno S  et al. miR-34c may protect lung cancer cells from paclitaxel-induced apoptosis. Oncogene : (2012). WB ; Human . Read more (PubMed: 22370637) »

    See all 3 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab32068.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册