概述

  • 产品名称Anti-Caspase-9抗体
    参阅全部 Caspase-9 一抗
  • 描述
    兔多克隆抗体to Caspase-9
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Non Human Primates
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-9 aa 100-200 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P55211
    (Peptide available as ab100814, ab100814)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: A431 - EGF-treated; HeLa; HepG2; HEK293; Jurkat.

性能

应用

Our Abpromise guarantee covers the use of ab90236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50, 46 kDa (predicted molecular weight: 46 kDa).

靶标

  • 功能Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • 组织特异性Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • 序列相似性Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 发展阶段Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • 翻译后修饰Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

Anti-Caspase-9 antibody 图像

  • All lanes : Anti-Caspase-9 antibody (ab90236) at 1 µg/ml

    Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate - EGF-treated
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 46,50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes
    We hypothesize that the 50 and 46 kDa bands represent the pro and mature forms of Caspase 9, respectively. Abcam welcomes customer feedback.

Anti-Caspase-9 antibody (ab90236)参考文献

ab90236 has not yet been referenced specifically in any publications.

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