Caspase 9 (active) Red Staining试剂盒(ab65619)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader, Fluor. microscope, Flow cyt.
- Assay time: 2 hr
- Sample type: Adherent cells, Suspension cells
概述
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产品名称
Caspase 9 (active) Red Staining试剂盒
参阅全部 Caspase-9 试剂盒 -
检测方法
Fluorescent -
样品类型
Adherent cells, Suspension cells -
检测类型
Enzyme activity -
检测时间
2h 00m -
产品概述
Abcam's Caspase 9 (active) Red Staining Kit provides a convenient means for detecting activated caspase 9 in living cells. The assay utilizes the caspase 9 inhibitor LEHD-FMK conjugated to sulfo-rhodamine (Red-LEHD-FMK) as the fluorescent in situ marker. Red-LEHD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase 9 in apoptotic cells.
Visit our FAQs page for tips and troubleshooting. -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K199 CaspGLOW™ Red Active Caspase-9 Staining Kit. K199-100 is the same size as the 100 test size of ab65619.
Activation of caspases plays a central role in apoptosis.
Other caspase and apoptosis assays
Review the full set of caspase assays, or the apoptosis assay and apoptosis marker guide.
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平台
Microplate reader, Fluor. microscope, Flow cyt.
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Red-LEHD-FMK 1 x 100µl Wash Buffer IV 2 x 100ml Z-VAD-FMK 1 x 10µl -
研究领域
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相关性
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. It is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 is approximately 47 kD. It is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. It becomes activated when recruited to the Apaf1/cytochrome c complex. -
别名
- APAF3
- CASP9
- Caspase 9
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数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab65619 被引用在 1 文献中.
- Shi LS et al. Effects of gastrokine-2 expression on gastric cancer cell apoptosis by activation of extrinsic apoptotic pathways. Mol Med Rep 10:2898-904 (2014). PubMed: 25270871