Caspase 9 (active) FITC Staining试剂盒(ab65615)

概述

  • 产品名称Caspase 9 (active) FITC Staining试剂盒
    参阅全部 Caspase 9 试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Enzyme activity
  • 检测时间
    2h 00m
  • 产品概述

    Abcam's Caspase 9 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase-9 in living cells. The assay utilizes the caspase-9 inhibitor LEHD-FMK conjugated to FITC (FITC-LEHD-FMK) as a fluorescent marker. FITC-LEHD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-9 in apoptotic cells.
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  • 说明

    Activation of caspases plays a central role in apoptosis.

  • 经测试应用适用于: FM, Functional Studies, Flow Cytmore details

性能

  • 相关性Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. It is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 is approximately 47 kD. It is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. It becomes activated when recruited to the Apaf1/cytochrome c complex.
  • 别名
    • APAF3
    • CASP9
    • Caspase 9
    • ICE LAP6
    • MCH6
    see all

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应用

Our Abpromise guarantee covers the use of ab65615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
FM Use at an assay dependent concentration.
Functional Studies Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.

实验方案

Caspase 9 (active) FITC Staining Kit (ab65615)参考文献

ab65615 has not yet been referenced specifically in any publications.

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