Caspase 8 Inhibitor Drug Detection试剂盒(ab102496)


  • 产品名称Caspase 8 Inhibitor Drug Detection试剂盒
    参阅全部 Caspase 8 试剂盒
  • 检测方法Fluorescent
  • 测试
    100 x 1 assay
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Quantitative
  • 检测时间
    1h 30m
  • 产品概述

    Abcam's Caspase 8 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate IETD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 8 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 8 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
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  • 说明

    Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.

  • 经测试应用适用于: Functional Studiesmore details
  • 平台Microplate


  • 相关性Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.
  • 细胞定位Cytoplasmic
  • 别名
    • Apoptotic cysteine protease
    • CAP4
    • CASP8
    • Caspase 8
    • CED 3
    • FLICE
    • MACH
    • MACH5
    see all


Our Abpromise guarantee covers the use of ab102496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Caspase 8 Inhibitor Drug Detection Kit 图像

  • Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using IETD-AFC as caspase 8 substrate and following Caspase 8 Inhibitor Drug Detection Kit (ab102496) protocol. Lanes:

    1.- Background Control: no Caspase 8, no z-VMAD-FMK

    2.- Positive Inhibition Control: no Caspase 8, +z-VAD-FMK

    3.- Positive Control: + Caspase 8, no z-VAD-FMK

    4.- Caspase 8 + 2.5µM z-VAD-FMK

    5.- Caspase 8 + 10µM z-VAD-FMK

    6.- Caspase 8 + 40µM z-VAD-FMK


Caspase 8 Inhibitor Drug Detection Kit (ab102496)参考文献

ab102496 has not yet been referenced specifically in any publications.

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