Caspase 8 Inhibitor Drug Detection试剂盒(ab102496)

概述

  • 产品名称
    Caspase 8 Inhibitor Drug Detection试剂盒
    参阅全部 Caspase 8 试剂盒
  • 检测方法
    Fluorescent
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型
    Quantitative
  • 检测时间
    1h 30m
  • 产品概述

    Abcam's Caspase 8 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate IETD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 8 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 8 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
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  • 说明

    Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.

  • 经测试应用
    适用于: Functional Studiesmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab102496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

图片

  • Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using IETD-AFC as caspase 8 substrate and following Caspase 8 Inhibitor Drug Detection Kit (ab102496) protocol. Lanes:

    1.- Background Control: no Caspase 8, no z-VMAD-FMK

    2.- Positive Inhibition Control: no Caspase 8, +z-VAD-FMK

    3.- Positive Control: + Caspase 8, no z-VAD-FMK

    4.- Caspase 8 + 2.5µM z-VAD-FMK

    5.- Caspase 8 + 10µM z-VAD-FMK

    6.- Caspase 8 + 40µM z-VAD-FMK

实验方案

文献

ab102496 has not yet been referenced specifically in any publications.

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