- 数据表
- 具体的参考文献 (28)
- 实验方案
概述
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产品名称Anti-Caspase-8抗体
参阅全部 Caspase-8 一抗 -
描述兔多克隆抗体to Caspase-8
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宿主Rabbit
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特异性ab25901 recognises the p18 form of Caspase-8.
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经测试应用适用于: ICC/IF, IHC-P, ICC, WBmore details
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种属反应性与反应: Mouse, Rat, Human
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免疫原
Synthetic peptide corresponding to Human Caspase-8 (C terminal). Synthetic peptide (Human) - a 16 amino acid peptide from near the carboxy-terminus of human Caspase-8 isoform A.
Database link: Q14790 -
阳性对照
- Jurkat cell lysate IHC-P: Human spleen FFPE tissue sections.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明Caspase-8 Antibody is affinity chromatography purified via peptide column.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Immunohistochemistry kits
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Positive Controls
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Related Products
应用
Our Abpromise guarantee covers the use of ab25901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| IHC-P | Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC | Use a concentration of 2 µg/ml. | |
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). |
靶标
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功能Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. -
组织特异性Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
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疾病相关Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.
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序列相似性Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains. -
结构域Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
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翻译后修饰Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位Cytoplasm.
- Information by UniProt
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数据库链接
- Entrez Gene: 841 Human
- Entrez Gene: 12370 Mouse
- Entrez Gene: 64044 Rat
- Omim: 601763 Human
- SwissProt: Q14790 Human
- SwissProt: O89110 Mouse
- SwissProt: Q9JHX4 Rat
- Unigene: 599762 Human
see all -
别名
- ALPS2B antibody
- Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody
- Apoptotic cysteine protease antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 antibody (ab25901)Immunocytochemistry/Immunofluorescence of Jurkat cells labeling Caspase-8 with Anti-Caspase-8 antibody (ab25901) at 20μg/ml. -
Western blot - Anti-Caspase-8 antibody (ab25901)Lane 1 : Anti-Caspase-8 antibody (ab25901) at 0.5 µg/ml
Lane 2 : Anti-Caspase-8 antibody (ab25901) at 1 µg/ml
Lane 3 : Anti-Caspase-8 antibody (ab25901) at 2 µg/ml
All lanes : Jurkat cell lysate.
Predicted band size: 18 kDa
Observed band size: 20 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry - Anti-Caspase-8 antibody (ab25901)Immunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 µg/ml.
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Western blot - Anti-Caspase-8 antibody (ab25901)All lanes : Anti-Caspase-8 antibody (ab25901) at 1 µg/ml
Lane 1 : Jurkat cell lysates with Absence of blocking buffer
Lane 2 : Jurkat cell lysates with Presence of blocking buffer
Predicted band size: 18 kDa -
Western blot - Anti-Caspase-8 antibody (ab25901)Anti-Caspase-8 antibody (ab25901) at 1 µg/ml +Recombinant human Caspase-8 protein (ab54117) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Exposure time: 1 minute -
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 antibody (ab25901)ab25901 at 2ug/ml staining Caspase-8 in Jurkat cells by ICC/IF
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab25901)IHC image of Caspase-8 staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25901, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
文献
This product has been referenced in:
- Li J et al. Ginsenoside Rg1 inhibits inflammatory responses via modulation of the nuclear factor-?B pathway and inhibition of inflammasome activation in alcoholic hepatitis. Int J Mol Med 41:899-907 (2018). IHC-P . Read more (PubMed: 29207044) »
- Yang J et al. Honokiol suppresses proliferation and induces apoptosis via regulation of the miR-21/PTEN/PI3K/AKT signaling pathway in human osteosarcoma cells. Int J Mol Med 41:1845-1854 (2018). Read more (PubMed: 29393336) »
客户评价及客户问答
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