Caspase 8 (active) Red Staining试剂盒(ab65618)

概述

  • 产品名称Caspase 8 (active) Red Staining试剂盒
    参阅全部 Caspase 8 试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Enzyme activity
  • 检测时间
    2h 00m
  • 产品概述

    Abcam's Caspase 8 (active) Red Staining Kit provides a convenient means for detecting activated caspase 8 in living cells. The assay utilizes the caspase 8 inhibitor IETD-FMK conjugated to sulfo-rhodamine (Red-IETD-FMK) as the fluorescent in situ marker. Red-IETD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase 8 in apoptotic cells.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Activation of caspases plays a central role in apoptosis.

  • 经测试应用适用于: FM, Functional Studies, Flow Cytmore details

性能

  • 相关性Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.
  • 细胞定位Cytoplasmic
  • 别名
    • Apoptotic cysteine protease
    • CAP4
    • CASP8
    • Caspase 8
    • CED 3
    • FLICE
    • MACH
    • MACH5
    see all

应用

Our Abpromise guarantee covers the use of ab65618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
FM Use at an assay dependent concentration.
Functional Studies Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.

实验方案

Caspase 8 (active) Red Staining Kit (ab65618)参考文献

ab65618 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab65618.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"