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Caspase 8 (active) FITC Staining Kit (ab65614) provides a convenient means for sensitive detection of activated caspase 8 in living cells. The assay utilizes the caspase 8 inhibitor, IETD-FMK, conjugated to FITC (FITC-IETD-FMK) as a marker. FITC-IETD-FMK is cell permeable, non-toxic, and irreversibly binds to activated caspase 8 in apoptotic cells. The FITC label allows detection of activated caspase-8 in apoptotic cells directly by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
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Activation of caspases plays a central role in apoptosis.
|FITC-IETD-FMK||1 x 100µl|
|Wash Buffer||2 x 100ml|
|Z-VAD-FMK||1 x 10µl|
Our Abpromise guarantee covers the use of ab65614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|FM||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent dilution.|
|Flow Cyt||Use at an assay dependent dilution.|
Active caspase 8 in Jurkat cells following four hours exposure to 50 ng/mL anti-Fas Ab (αFas) (MBL), five hours with 10 μg/mL cyclohexamide (CHX) (ab120093), or one hour pretreatment with CHX followed by four hours with αFas. Background signal subtracted, duplicates; +/- SD.
Image obtained from Hernandez-Flores G et al; BMC Cancer, 2011 Nov 11; 11:483
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