The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
1/50 - 1/100.
应用说明Is unsuitable for Flow Cyt.
功能Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Overexpression promotes programmed cell death.
组织特异性Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
序列相似性Belongs to the peptidase C14A family.
翻译后修饰Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
Immunofluorescent staining of HeLa cells using ab32522 at 1:100 dilution.
Anti-Caspase-7 antibody [E22] (ab32522)参考文献
This product has been referenced in:
Wang X et al. Synergistic control of sex hormones by 17ß-HSD type 7: a novel target for estrogen-dependent breast cancer. J Mol Cell Biol7:568-79 (2015).
Read more (PubMed: 25966904) »
Edelmann B et al. Caspase-8 and caspase-7 sequentially mediate proteolytic activation of acid sphingomyelinase in TNF-R1 receptosomes. EMBO J30:379-94 (2011).
Read more (PubMed: 21157428) »