概述

  • 产品名称Anti-Caspase-7抗体[E22]
    参阅全部 Caspase-7 一抗
  • 描述
    兔单克隆抗体[E22] to Caspase-7
  • 特异性The antibody should recognize both pro-form and p20 cleaved-form. The antibody does not cross-react with other Caspase family members.
  • 经测试应用适用于: WB, IHC-P, ICC/IF, IPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caspase-7 aa 1-100 (N terminal).
    Database link: P55210

  • 阳性对照
    • Jurkat cell lysate, human skin cancer tissue and HeLa cells.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Caspase-7 antibody (HRP) [E22] (ab206039)

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab32522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100.
IP 1/10.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
      -Gly-217' bond. Overexpression promotes programmed cell death.
    • 组织特异性Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
    • 序列相似性Belongs to the peptidase C14A family.
    • 翻译后修饰Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Apoptotic protease Mch-3 antibody
      • Apoptotic protease MCH3 antibody
      • CASP-7 antibody
      • CASP7 antibody
      • CASP7_HUMAN antibody
      • Caspase 7 antibody
      • Caspase 7 apoptosis related cysteine peptidase antibody
      • Caspase-7 subunit p11 antibody
      • Caspase7 antibody
      • CMH 1 antibody
      • CMH-1 antibody
      • CMH1 antibody
      • ICE LAP3 antibody
      • ICE-LAP3 antibody
      • ICE-like apoptotic protease 3 antibody
      • LICE2 antibody
      • MCH3 antibody
      see all

    Anti-Caspase-7 antibody [E22] 图像

    • Anti-Caspase-7 antibody [E22] (ab32522) at 1/1000 dilution + Jurkat cell lysate

      Predicted band size : 34 kDa
      Observed band size : 34 kDa
    • Immunohistochemical analysis of paraffin embedded human skin cancer tissue using ab32522 at 1:50 dilution.

    • Immunofluorescent staining of HeLa cells using ab32522 at 1:100 dilution.

    Anti-Caspase-7 antibody [E22] (ab32522)参考文献

    This product has been referenced in:
    • Wang X  et al. Synergistic control of sex hormones by 17ß-HSD type 7: a novel target for estrogen-dependent breast cancer. J Mol Cell Biol 7:568-79 (2015). Read more (PubMed: 25966904) »
    • Edelmann B  et al. Caspase-8 and caspase-7 sequentially mediate proteolytic activation of acid sphingomyelinase in TNF-R1 receptosomes. EMBO J 30:379-94 (2011). WB, ICC/IF ; Human . Read more (PubMed: 21157428) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Immuno-precipitation step Staph. aureus
    Sample Human Cell lysate - whole cell (MCF7)
    Specification MCF7
    Total protein in input 100 µg
    Username

    Abcam user community

    Verified customer

    提交于 Sep 05 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (10)
    Sample Human Cell lysate - whole cell (MCF7)
    Specification MCF7
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 05 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Immuno-precipitation step Protein A/G
    Sample Human Cell lysate - whole cell (MCF7)
    Specification MCF7
    Total protein in input 100 µg
    Username

    Abcam user community

    Verified customer

    提交于 Jul 05 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 100 µg
    Gel Running Conditions Reduced Denaturing (8)
    Sample Human Cell lysate - whole cell (MCF7)
    Specification MCF7
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 04 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate
    Sample Human Tissue sections (human breast cancer tissue)
    Specification human breast cancer tissue
    Permeabilization Yes - xylene
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jun 28 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (10)
    Sample Human Cell lysate - whole cell (MCF7)
    Specification MCF7
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 18 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (MCF7)
    Total protein in input 100 µg
    Immuno-precipitation step Other - sepharose beads
    Specification MCF7
    Username

    Abcam user community

    Verified customer

    提交于 Jun 17 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"