概述

  • 产品名称
    Anti-Caspase-7抗体
    参阅全部 Caspase-7 一抗
  • 描述
    兔多克隆抗体to Caspase-7
  • 特异性
    The immunogen used for this product detects both caspase-7 precursor and small 105 aa C-terminal p12/12 kDa subunit in staurosporine (STS) treated cells.
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-7 aa 250 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P55210
    (Peptide available as ab102735)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate; Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate; HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

性能

应用

Our Abpromise guarantee covers the use of ab92842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 mg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能
    Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Overexpression promotes programmed cell death.
  • 组织特异性
    Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
  • 序列相似性
    Belongs to the peptidase C14A family.
  • 翻译后修饰
    Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apoptotic protease Mch-3 antibody
    • Apoptotic protease MCH3 antibody
    • CASP-7 antibody
    • CASP7 antibody
    • CASP7_HUMAN antibody
    • Caspase 7 antibody
    • Caspase 7 apoptosis related cysteine peptidase antibody
    • Caspase-7 subunit p11 antibody
    • Caspase7 antibody
    • CMH 1 antibody
    • CMH-1 antibody
    • CMH1 antibody
    • ICE LAP3 antibody
    • ICE-LAP3 antibody
    • ICE-like apoptotic protease 3 antibody
    • LICE2 antibody
    • MCH3 antibody
    see all

图片



  • Predicted band size : 34 kDa

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Wild-type HAP1 treated with Staurosporin whole cell lysate (20 µg)
    Lane 3: Caspase-7 knockout HAP1 whole cell lysate (20 µg)
    Lane 4: Caspase-7 knockout HAP1 treated with Staurosporin whole cell lysate (20 µg)
    Lane 5: HeLa whole cell lysate (20 µg)
    Lane 6: HeLa treated with Staurosporin whole cell lysate (20 µg)

    Lanes 1 - 6: Merged signal (red and green). Green - ab92842 observed at 37, 12 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab92842 was shown to recognize Caspase-7 in wild-type cells as signal was lost at the expected MW in Caspase-7 knockout cells. Additional cross-reactive bands were observed in the wild-type and Caspase-7 knockout cells. Wild-type and Caspase-7 knockout samples were subjected to SDS-PAGE. Ab92842 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-Caspase-7 antibody (ab92842) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 17 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

    We hypothesize that the 15 kDa band represents the cleaved subunit (P11) of Caspase-7. Abcam welcomes customer feedback.

  • ICC/IF image of ab92845 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92842, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1:1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml.

文献

ab92842 has not yet been referenced specifically in any publications.

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