Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Protein A purified
ab63440 was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
1/500 - 1/1000.
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
Belongs to the peptidase C14A family.
Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
Information by UniProt
Apoptotic protease Mch-2 antibody
Apoptotic protease MCH2 antibody
Anti-Caspase-6 (phospho S257) antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-6 (phospho S257) antibody (ab63440)
ab63440 at 1:500 - 1:1000 dilution staining Caspase-6 in HuvEc cells by Immunofluorescence, in the absence or presence of the immunising peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6 (phospho S257) antibody (ab63440)
ab63440 at 1:50 - 1:100 dilution staining Caspase-6 in human colon carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
Western blot - Anti-Caspase-6 (phospho S257) antibody (ab63440)
All lanes :
Anti-Caspase-6 (phospho S257) antibody (ab63440) at 1/500 dilution
Lane 1 :
293 cell extracts treated
with Etoposide (25uM, 60min)
Lane 2 :
293 cell extracts treated
with Etoposide (25uM, 60min) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size :
Observed band size :
36 kDa (
why is the actual band size different from the predicted?
The amount of positive control loading is generally 5-30 ug of total protein. The amount of the peptide is 5-10 ug.
Anti-Caspase-6 (phospho S257) antibody (ab63440)参考文献
has not yet been referenced specifically in any publications.
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