Anti-Caspase-6 (phospho S257)抗体(ab63440)

概述

  • 产品名称Anti-Caspase-6 (phospho S257)抗体
    参阅全部 Caspase-6 一抗
  • 描述
    兔多克隆抗体to Caspase-6 (phospho S257)
  • 特异性ab63440 detects endogenous levels of Caspase-6 only when phosphorylated at serine 257.
  • 经测试应用适用于: ELISA, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-6.
    Database link: P55212

  • 阳性对照
    • Human colon carcinoma tissue, HuvEc cells and 293 cell extracts treated with Etoposide (25uM, 60min).

性能

应用

Our Abpromise guarantee covers the use of ab63440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
ICC/IF 1/500 - 1/1000.

靶标

  • 功能Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
  • 序列相似性Belongs to the peptidase C14A family.
  • 翻译后修饰Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apoptotic protease Mch-2 antibody
    • Apoptotic protease MCH2 antibody
    • CASP-6 antibody
    • CASP6 antibody
    • CASP6_HUMAN antibody
    • Caspase 6 antibody
    • Caspase 6 apoptosis related cysteine protease antibody
    • Caspase 6, apoptosis related cysteine peptidase antibody
    • Caspase-6 subunit p11 antibody
    • Mch2 antibody
    • OTTHUMP00000162742 antibody
    • OTTHUMP00000162743 antibody
    see all

Anti-Caspase-6 (phospho S257) antibody 图像

  • ab63440 at 1:500 - 1:1000 dilution staining Caspase-6 in HuvEc cells by Immunofluorescence, in the absence or presence of the immunising peptide.

  • ab63440 at 1:50 - 1:100 dilution staining Caspase-6 in human colon carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

  • All lanes : Anti-Caspase-6 (phospho S257) antibody (ab63440) at 1/500 dilution

    Lane 1 : 293 cell extracts treated with Etoposide (25uM, 60min)
    Lane 2 : 293 cell extracts treated with Etoposide (25uM, 60min) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 33 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    The amount of positive control loading is generally 5-30 ug of total protein. The amount of the peptide is 5-10 ug.

Anti-Caspase-6 (phospho S257) antibody (ab63440)参考文献

ab63440 has not yet been referenced specifically in any publications.

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