Caspase 6 Inhibitor Drug Detection试剂盒(ab102494)

概述

  • 产品名称Caspase 6 Inhibitor Drug Detection试剂盒
    参阅全部 Caspase 6 试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Quantitative
  • 检测时间
    1h 30m
  • 产品概述

    Abcam's Caspase 6 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate VEID-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 6 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 6 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
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  • 说明

    Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Caspase 6 is involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Caspase 3 and Caspase 6 are the major active caspases in apoptotic cells, and are activated in response to distinct apoptosis inducing stimuli and in all cell lines analyzed. Both Caspase 3 and 6 are present in apoptotic cells as multiple active species. Caspase 6 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei.
  • 细胞定位Cytoplasmic
  • 别名
    • Apoptotic Protease MCH2
    • CASP6
    • Caspase 6 apoptosis related cysteine protease
    • Caspase 6 precursor
    • MCH2
    see all

相关产品

应用

Our Abpromise guarantee covers the use of ab102494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

实验方案

Caspase 6 Inhibitor Drug Detection Kit (ab102494)参考文献

ab102494 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry. This kit is generally used with cell lysates, but theoretically it could be used for blood and other samples. This kit is intended for research use only because it is not validated for patient clinical samples. It is ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"