Caspase 6 Assay试剂盒(Fluorometric) (ab39707)

概述

  • 产品名称Caspase 6 Assay试剂盒(Fluorometric)
    参阅全部 Caspase 6 试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Tissue Extracts, Cell Lysate
  • 检测类型Enzyme activity
  • 检测时间
    2h 00m
  • 产品概述

    Abcam's Caspase 6 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VEID. The assay is based on detection of cleavage of substrate VEID-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VEID-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase 6 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader.
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  • 说明

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Caspase 6 is involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Caspase 3 and Caspase 6 are the major active caspases in apoptotic cells, and are activated in response to distinct apoptosis inducing stimuli and in all cell lines analyzed. Both Caspase 3 and 6 are present in apoptotic cells as multiple active species. Caspase 6 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei.
  • 细胞定位Cytoplasmic
  • 别名
    • Apoptotic Protease MCH2
    • CASP6
    • Caspase 6 apoptosis related cysteine protease
    • Caspase 6 precursor
    • MCH2
    see all

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应用

Our Abpromise guarantee covers the use of ab39707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

实验方案

Caspase 6 Assay Kit (Fluorometric) (ab39707)参考文献

ab39707 has not yet been referenced specifically in any publications.

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