RabMAb

Anti-Caspase-6抗体[EPR4405] (ab108335)

概述

  • 产品名称Anti-Caspase-6抗体[EPR4405]
    参阅全部 Caspase-6 一抗
  • 描述
    兔单克隆抗体[EPR4405] to Caspase-6
  • 特异性ab108335 detects both the full length and cleaved forms of Caspase-6.
  • 经测试应用适用于: WB, IP, Flow Cytmore details
    不适用于: ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caspase-6 aa 150-250.
    Database link: P55212

  • 阳性对照
    • Jurkat cells and cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab108335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC or IHC-P.
  • 靶标

    • 功能Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
    • 序列相似性Belongs to the peptidase C14A family.
    • 翻译后修饰Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Apoptotic protease Mch-2 antibody
      • Apoptotic protease MCH2 antibody
      • CASP-6 antibody
      • CASP6 antibody
      • CASP6_HUMAN antibody
      • Caspase 6 antibody
      • Caspase 6 apoptosis related cysteine protease antibody
      • Caspase 6, apoptosis related cysteine peptidase antibody
      • Caspase-6 subunit p11 antibody
      • Mch2 antibody
      • OTTHUMP00000162742 antibody
      • OTTHUMP00000162743 antibody
      see all

    Anti-Caspase-6 antibody [EPR4405] 图像

    • All lanes : Anti-Caspase-6 antibody [EPR4405] (ab108335) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Jurkat + staurosporine cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 33 kDa
    • ab108335, at 1:10 dilution, staining Caspase-6 in permeabilized Jurkat cells by Flow cytometry (shown in red). Rabbit IgG shown in green (negative).

    Anti-Caspase-6 antibody [EPR4405] (ab108335)参考文献

    ab108335 has not yet been referenced specifically in any publications.

    Product Wall

    Application Flow Cytometry
    Fixation Formaldehyde
    Permeabilization Yes - 0.1% PBS-Tween
    Sample Mouse Cell (ledyig cells)
    Specification ledyig cells
    Gating Strategy RLF positive cells
    Preparation Cell harvesting/tissue preparation method: Tubules from testes were incubated for 15–20 min at 35¯C in a shaking water bath at 80 oscillations (osc)/min , then layered over 40 mL 5% Percoll and leydig cells were isolated from top of the percoll
    Sample buffer: 0.1% PBS BSA
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    提交于 Dec 04 2013

    Application Flow Cytometry
    Fixation Methanol
    Permeabilization Yes - 0.1% PBS-Tween
    Sample Rat Cell (breast cells)
    Specification breast cells
    Gating Strategy cd1d1 POSITIVE
    Preparation Cell harvesting/tissue preparation method: tissue was treated with collagenase to make single cell suspension
    Sample buffer: 0.1% PBS BSA
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    提交于 Dec 04 2013

    Application Flow Cytometry
    Fixation Methanol
    Permeabilization Yes - 0.1% PBS-Tween
    Sample Human Cell (MCF7)
    Specification MCF7
    Gating Strategy CD44 POSITIVE
    Preparation Cell harvesting/tissue preparation method: treated with collagenase and trypsin to make single cell suspension
    Sample buffer: 0.1% PBS BSA
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    提交于 Dec 04 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Immuno-precipitation step Staph. aureus
    Sample Human Cell lysate - whole cell (MCF7)
    Specification MCF7
    Total protein in input 100 µg
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    提交于 Sep 09 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (10)
    Sample Human Cell lysate - whole cell (MCF7)
    Specification MCF7
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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    提交于 Sep 06 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Immuno-precipitation step Staph. aureus
    Sample Human Cell lysate - whole cell (mcf7)
    Specification mcf7
    Total protein in input 50 µg
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    Verified customer

    提交于 Jun 27 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"