重组
RabMAb

Anti-Caspase-6抗体[EPR4405] (ab108335)

概述

  • 产品名称
    Anti-Caspase-6抗体[EPR4405]
    参阅全部 Caspase-6 一抗
  • 描述
    兔单克隆抗体[EPR4405] to Caspase-6
  • 特异性
    ab108335 detects both the full length and cleaved forms of Caspase-6.
  • 经测试应用
    适用于: WB, IP, Flow Cytmore details
    不适用于: ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caspase-6 aa 150-250.
    Database link: P55212

  • 阳性对照
    • Jurkat cells and cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab108335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明
    Is unsuitable for ICC or IHC-P.
  • 靶标

    • 功能
      Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
    • 序列相似性
      Belongs to the peptidase C14A family.
    • 翻译后修饰
      Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
    • 细胞定位
      Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Apoptotic protease Mch-2 antibody
      • Apoptotic protease MCH2 antibody
      • CASP-6 antibody
      • CASP6 antibody
      • CASP6_HUMAN antibody
      • Caspase 6 antibody
      • Caspase 6 apoptosis related cysteine protease antibody
      • Caspase 6, apoptosis related cysteine peptidase antibody
      • Caspase-6 subunit p11 antibody
      • Mch2 antibody
      • OTTHUMP00000162742 antibody
      • OTTHUMP00000162743 antibody
      see all

    图片

    • All lanes : Anti-Caspase-6 antibody [EPR4405] (ab108335) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Jurkat + staurosporine cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 33 kDa
    • ab108335, at 1:10 dilution, staining Caspase-6 in permeabilized Jurkat cells by Flow cytometry (shown in red). Rabbit IgG shown in green (negative).

    文献

    This product has been referenced in:
    • Ma J  et al. Reversing drug resistance of soft tumor-repopulating cells by tumor cell-derived chemotherapeutic microparticles. Cell Res 26:713-27 (2016). Read more (PubMed: 27167569) »

    See 1 Publication for this product

    客户评价及客户问答

    Application
    Flow Cytometry
    Fixation
    Formaldehyde
    Permeabilization
    Yes - 0.1% PBS-Tween
    Sample
    Mouse Cell (ledyig cells)
    Specification
    ledyig cells
    Gating Strategy
    RLF positive cells
    Preparation
    Cell harvesting/tissue preparation method: Tubules from testes were incubated for 15–20 min at 35¯C in a shaking water bath at 80 oscillations (osc)/min , then layered over 40 mL 5% Percoll and leydig cells were isolated from top of the percoll
    Sample buffer: 0.1% PBS BSA
    Username

    Abcam user community

    Verified customer

    提交于 Dec 04 2013

    Application
    Flow Cytometry
    Fixation
    Methanol
    Permeabilization
    Yes - 0.1% PBS-Tween
    Sample
    Rat Cell (breast cells)
    Specification
    breast cells
    Gating Strategy
    cd1d1 POSITIVE
    Preparation
    Cell harvesting/tissue preparation method: tissue was treated with collagenase to make single cell suspension
    Sample buffer: 0.1% PBS BSA
    Username

    Abcam user community

    Verified customer

    提交于 Dec 04 2013

    Application
    Flow Cytometry
    Fixation
    Methanol
    Permeabilization
    Yes - 0.1% PBS-Tween
    Sample
    Human Cell (MCF7)
    Specification
    MCF7
    Gating Strategy
    CD44 POSITIVE
    Preparation
    Cell harvesting/tissue preparation method: treated with collagenase and trypsin to make single cell suspension
    Sample buffer: 0.1% PBS BSA
    Username

    Abcam user community

    Verified customer

    提交于 Dec 04 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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