概述

  • 产品名称Anti-Caspase-6抗体[E180]
    参阅全部 Caspase-6 一抗
  • 描述
    兔单克隆抗体[E180] to Caspase-6
  • 特异性This antibody detects the pro form and the p11 subunit of human Caspase-6. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caspase-6 aa 250-350 (C terminal).
    Database link: P55212

  • 阳性对照
    • WB: Jurkat cell lysate. ICC/IF: Hela cells. IHC-P: Human lung carcinoma tissue slides.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

性能

应用

Our Abpromise guarantee covers the use of ab32366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
    • 序列相似性Belongs to the peptidase C14A family.
    • 翻译后修饰Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Apoptotic protease Mch-2 antibody
      • Apoptotic protease MCH2 antibody
      • CASP-6 antibody
      • CASP6 antibody
      • CASP6_HUMAN antibody
      • Caspase 6 antibody
      • Caspase 6 apoptosis related cysteine protease antibody
      • Caspase 6, apoptosis related cysteine peptidase antibody
      • Caspase-6 subunit p11 antibody
      • Mch2 antibody
      • OTTHUMP00000162742 antibody
      • OTTHUMP00000162743 antibody
      see all

    Anti-Caspase-6 antibody [E180] 图像

    • Ab32366, at a 1:50 dilution, staining Caspase-6 in paraffin embedded human lung adenocarcinoma tissue by Immunohistochemistry.

    • Ab32366, at a 1:100 dilution, staining Caspase-6 in Hela cells by Immunocytochemistry/Immunofluorescence.

    • All lanes : Anti-Caspase-6 antibody [E180] (ab32366) at 1/1000 dilution

      Lane 1 : Untreated Jurkat cell lysate.
      Lane 2 : Jurkat cell lysate treated with Camptothecin.


      Predicted band size : 34 kDa
      Observed band size : 30 kDa (why is the actual band size different from the predicted?)

    Anti-Caspase-6 antibody [E180] (ab32366)参考文献

    This product has been referenced in:

    See 1 Publication for this product

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