产品名称 Anti-Caspase-6抗体 参阅全部 Caspase-6 一抗
特异性 ab63487 detects endogenous levels of total Caspase-6 protein.
ELISA, IHC-P, WB, ICC/IF more details
Synthetic peptide corresponding to Human Caspase-6. Immunogen is within aa from 223-272.
Database link: P55212
IHC-P: Human breast carcinoma tissue
ICC/IF: HuvEc cells
WB: A431 cell extracts
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 ab63487 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/500 - 1/1000. Predicted molecular weight: 33 kDa.
1/500 - 1/1000.
功能 Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
序列相似性 Belongs to the peptidase C14A family.
翻译后修饰 Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
Information by UniProt
Apoptotic protease Mch-2 antibody
Apoptotic protease MCH2 antibody
Anti-Caspase-6 antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-6 antibody (ab63487)
ab63487, at 1/500 dilution, staining Caspase-6 in HuvEc cells by Immunofluorescence, in the absence (left image) or presence (right image) of the immunising peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6 antibody (ab63487)
ab63487, at 1:50 dilution, staining Caspase-6 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.
Western blot - Anti-Caspase-6 antibody (ab63487)
All lanes : Anti-Caspase-6 antibody (ab63487) at 1/500 dilution Lane 1 : A431 cell extracts Lane 2 : A431 cell extracts with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 33 kDa Observed band size : 33 kDa Additional bands at : 25 kDa,30 kDa. We are unsure as to the identity of these extra bands.
Anti-Caspase-6 antibody (ab63487)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"