Caspase 5 Assay试剂盒(Fluorometric) (ab65660)

概述

  • 产品名称Caspase 5 Assay试剂盒(Fluorometric)
    参阅全部 Caspase 5 试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Tissue Extracts, Cell Lysate
  • 检测类型Enzyme activity
  • 检测时间
    2h 00m
  • 产品概述

    Abcam's Caspase 5 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence WEHD. The assay is based on detection of cleavage of substrate WEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). WEHD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase 5 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.
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  • 说明

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Caspase 5 belongs to the Ced3/ICE family of cysteine proteases. Caspase 5 exists as an inactive proenzyme which undergoes proteolytic processing at conserved aspartic residues, leading to p20 and p10 subunits that dimerize to form the active enzyme. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.
  • 别名
    • CASP 5
    • CASP5
    • Caspase 5 apoptosis related cysteine peptidase
    • Caspase5
    • ICE(rel) III
    • ICE(rel)III
    • ICEREL III
    • ICERELIII
    • ICH 3
    • ICH 3 protease
    • ICH3
    • MGC141966
    • TY protease
    see all

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应用

Our Abpromise guarantee covers the use of ab65660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

实验方案

Caspase 5 Assay Kit (Fluorometric) (ab65660)参考文献

ab65660 has not yet been referenced specifically in any publications.

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