Caspase 5 Assay试剂盒(Colorimetric) (ab65661)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Tissue Extracts
概述
-
产品名称
Caspase 5 Assay试剂盒(Colorimetric)
参阅全部 Caspase-5 试剂盒 -
检测方法
Colorimetric -
样品类型
Tissue Extracts, Cell Lysate -
检测类型
Enzyme activity -
检测时间
2h 00m -
产品概述
Abcam's Caspase 5 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence WEHD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate WEHD-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
Visit our FAQs page for tips and troubleshooting. -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K123 Caspase-5 Colorimetric Assay Kit. K123-100 is the same size as the 100 test size of ab65661.
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
Other caspase and apoptosis assays
Review the full set of caspase assays, or the apoptosis assay and apoptosis marker guide.
-
平台
Microplate reader
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests 2X Reaction Buffer 4 x 2ml Cell Lysis Buffer 1 x 100ml Dilution Buffer II 1 x 100ml DTT 1 x 400µl WEHD-pNA (4mM) 1 x 500µl -
研究领域
-
相关性
Caspase 5 belongs to the Ced3/ICE family of cysteine proteases. Caspase 5 exists as an inactive proenzyme which undergoes proteolytic processing at conserved aspartic residues, leading to p20 and p10 subunits that dimerize to form the active enzyme. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. -
别名
- CASP 5
- CASP5
- Caspase 5 apoptosis related cysteine peptidase
see all
相关产品
-
Related Products
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab65661 尚未被引用在任何文献中。