Anti-Caspase 5抗体[CAS5] (ab10448)

概述

  • 产品名称Anti-Caspase 5抗体[CAS5]
    参阅全部 Caspase 5 一抗
  • 描述
    小鼠单克隆抗体[CAS5] to Caspase 5
  • 经测试应用适用于: ELISA, WB, Double Immunodiffusionmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    GEKHGELWVR

    conjugated to KLH, corresponding to amino acids 300-311 of Human Caspase 5.

  • 表位The epitope recognized by the antibody resides within amino acids 300-311 of human Caspase 5 molecule.
  • 阳性对照
    • Whole extract of HeLa cells activated with lipopolysaccharide (LPS).
  • 常规说明For continuous use, store at 2-8 °C for up to one month. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号CAS5
  • 同种型IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
Double Immunodiffusion
  • 应用说明DID: Use at an assay dependent dilution.
    ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 5 - 10 µg/ml (determined by using a whole extract of HeLa cells activated with lipopolysaccharide (LPS)). Expected bands: a doublet at approx. 42 kDa, with additional bands at approx. 75 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Mediator of programmed cell death (apoptosis).
    • 组织特异性Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
    • 序列相似性Belongs to the peptidase C14A family.
      Contains 1 CARD domain.
    • 翻译后修饰The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Apoptosis related cysteine protease antibody
      • CASP-5 antibody
      • CASP5 antibody
      • CASP5_HUMAN antibody
      • Caspase-5 subunit p10 antibody
      • ICE(rel)-III antibody
      • ICERELIII antibody
      • ICH 3 antibody
      • ICH 3 protease antibody
      • Protease ICH-3 antibody
      • Protease TY antibody
      • TY antibody
      • TY protease antibody
      see all

    Anti-Caspase 5 antibody [CAS5] (ab10448)参考文献

    ab10448 has not yet been referenced specifically in any publications.

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