概述

  • 产品名称Anti-Caspase 5抗体
    参阅全部 Caspase 5 一抗
  • 描述
    兔多克隆抗体to Caspase 5
  • 特异性ab69641 can detect a fragment of activated Caspase 5 resulting from cleavage adjacent to Asp121.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthesized peptide derived from human Caspase 5

  • 阳性对照
    • Extracts from HeLa cells and A549 cells, treated with etoposide (25uM, 24hours).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab69641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: 1/20000.
    WB: 1/500 - 1/1000. Detects a band of approximately 26 and 48 kDa (predicted molecular weight of precursor): 48 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Mediator of programmed cell death (apoptosis).
    • 组织特异性Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
    • 序列相似性Belongs to the peptidase C14A family.
      Contains 1 CARD domain.
    • 翻译后修饰The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Apoptosis related cysteine protease antibody
      • CASP-5 antibody
      • CASP5 antibody
      • CASP5_HUMAN antibody
      • Caspase-5 subunit p10 antibody
      • ICE(rel)-III antibody
      • ICERELIII antibody
      • ICH 3 antibody
      • ICH 3 protease antibody
      • Protease ICH-3 antibody
      • Protease TY antibody
      • TY antibody
      • TY protease antibody
      see all

    Anti-Caspase 5 antibody 图像

    • All lanes : Anti-Caspase 5 antibody (ab69641) at 1/500 dilution

      Lane 1 : HeLa cells treated with etoposide (25uM, 24hours)
      Lane 2 : A549 cells treated with etoposide (25uM, 24hours)
      Lane 3 : HeLa cells treated with etoposide (25uM, 24hours) with immunising peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 48 kDa
      Observed band size : 26,48 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 26 kDa (possible cleavage fragment).

    Anti-Caspase 5 antibody (ab69641)参考文献

    ab69641 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab69641.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"