产品名称 Anti-Caspase 5抗体 参阅全部 Caspase 5 一抗
兔多克隆抗体to Caspase 5
特异性 ab69641 can detect a fragment of activated Caspase 5 resulting from cleavage adjacent to Asp121.
WB, ELISA more details
A synthesized peptide derived from human Caspase 5
Extracts from HeLa cells and A549 cells, treated with etoposide (25uM, 24hours).
存放说明 Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明 ELISA: 1/20000.
WB: 1/500 - 1/1000. Detects a band of approximately 26 and 48 kDa (predicted molecular weight of precursor): 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能 Mediator of programmed cell death (apoptosis).
组织特异性 Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
序列相似性 Belongs to the peptidase C14A family. Contains 1 CARD domain.
翻译后修饰 The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
Information by UniProt
Apoptosis related cysteine protease antibody
Anti-Caspase 5 antibody 图像
Western blot - Caspase 5 antibody (ab69641)
All lanes : Anti-Caspase 5 antibody (ab69641) at 1/500 dilution Lane 1 : HeLa cells treated with etoposide (25uM, 24hours) Lane 2 :
A549 cells treated with etoposide (25uM, 24hours) Lane 3 : HeLa cells treated with etoposide (25uM, 24hours) with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 48 kDa Observed band size : 26,48 kDa ( why is the actual band size different from the predicted?) Additional bands at : 26 kDa (possible cleavage fragment).
Anti-Caspase 5 antibody (ab69641)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"