Caspase 4 Assay试剂盒(Fluorometric) (ab65658)

概述

  • 产品名称Caspase 4 Assay试剂盒(Fluorometric)
    参阅全部 Caspase 4 试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Tissue Extracts, Cell Lysate
  • 检测类型Enzyme activity
  • 检测时间
    2h 00m
  • 产品概述

    Abcam's Caspase 4 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on detection of cleavage of substrate LEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEVD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase 4 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.
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  • 说明

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Caspase 4 is a member of the cysteine-aspartic acid protease (caspase) family. It is involved in the activation cascade of caspases responsible for apoptosis execution. Caspases exist as inactive proenzymes. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of large and small subunits. Caspase 4 is able to cleave and activate its own precursor protein, as well as the caspase 1 precursor. Caspase 4 mRNA is found in most tissues examined with the exception of the brain. Highest expression is found in spleen and lung, with moderate expression in heart and liver. Low expression is observed with skeletal muscle, kidney, and testis.
  • 细胞定位Cytoplasmic and Mitochondrial
  • 别名
    • CASP4
    • Caspase4
    • ICE(rel)II
    • ICERELII
    • ICH2
    • TX
    see all

应用

Our Abpromise guarantee covers the use of ab65658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

实验方案

Caspase 4 Assay Kit (Fluorometric) (ab65658)参考文献

ab65658 has not yet been referenced specifically in any publications.

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