概述

  • 产品名称
    Anti-Caspase-3抗体
    参阅全部 Caspase-3 一抗
  • 描述
    兔多克隆抗体to Caspase-3
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Saccharomyces cerevisiae, Monkey
  • 免疫原

    Recombinant full length protein corresponding to Human Caspase-3 aa 1-277.
    Sequence:

    MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIII NNKNFHKSTG MTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVE LMRDVSKEDHSKRSSFVCVLLS HGEEGIIFGTNGPVDLKKITNFFRGD RCRSLTGKPKLFIIQACRGTELDCGIETDSGVDD DMACHKIPVEADFL YAYSTAPGYYSWRNSKDGSWFIQSLCAMLKQYADKLEFMHILTRVN RK VATEFESFSFDATFHAKKQIPCIVSMLTKELYFYH


    Database link: P42574

  • 阳性对照
    • Jurkat cell lysate, Jurkat cells treated with staurosporine (lysate), HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab90437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 32 kDa.

A lower molecular weight band refers to a proteolytic cleavage product of caspase-3.

IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • 组织特异性
    Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • 序列相似性
    Belongs to the peptidase C14A family.
  • 翻译后修饰
    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A830040C14Rik antibody
    • Apopain antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Casp3a antibody
    • Caspase 3 antibody
    • Caspase 3, apoptosis-related cysteine peptidase antibody
    • Caspase 3, apoptosis-related cysteine protease antibody
    • Caspase 3, apoptosis-related cysteine protease a antibody
    • Caspase-3 subunit p12 antibody
    • CC3 antibody
    • CPP-32 antibody
    • CPP32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • EC 3.4.22.56 antibody
    • LICE antibody
    • mldy antibody
    • OTTHUMP00000165052 antibody
    • OTTHUMP00000165053 antibody
    • OTTHUMP00000165054 antibody
    • PARP cleavage protease antibody
    • Procaspase3 antibody
    • Protein Yama antibody
    • SCA 1 antibody
    • SCA-1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    see all

图片

  • All lanes : Anti-Caspase-3 antibody (ab90437) at 1/1000 dilution

    Lane 1 : Mouse hepatocytes whole cell lysate poly(I:C) treated - 0 hours
    Lane 2 : Mouse hepatocytes whole cell lysate poly(I:C) treated - 12 hours

    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 32 kDa


    Exposure time : 20 seconds

    This image is courtesy of an anonymous abreview.

    Blocking with 5% milk for 1 hour at 23°C.

    See Abreview

  • Immunohistochemistry analysis of human spleen tissue stained with Caspase-3, pAb at 10µg/ml.

  • All lanes : Anti-Caspase-3 antibody (ab90437) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cells treated with staurosporine
    Lane 3 : MCF-7 cell lysate (negative control)

    Developed using the ECL technique

    Predicted band size : 32 kDa
    Additional bands at : ~18 kDa (possible cleavage fragment),~20 kDa (possible cleavage fragment).

    Western blot analysis of Caspase-3 pAb.

文献

This product has been referenced in:
  • Zhang C  et al. Growth of tyrosine kinase inhibitor-resistant Philadelphia-positive acute lymphoblastic leukemia: Role of bone marrow stromal cells. Oncol Lett 13:2059-2070 (2017). WB . Read more (PubMed: 28454362) »
  • Wiese M  et al. The ß-catenin/CBP-antagonist ICG-001 inhibits pediatric glioma tumorigenicity in a Wnt-independent manner. Oncotarget 8:27300-27313 (2017). Read more (PubMed: 28460484) »

See all 16 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue sections (Neural tube)
Permeabilization
No
Specification
Neural tube
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Ayelet Kohl

Verified customer

提交于 Sep 21 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 29 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 29 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Chicken Cell (Embryo)
Permeabilization
Yes - 0.1%Tween
Specification
Embryo
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

Maneeshi Prasad

Verified customer

提交于 Oct 08 2015

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
20 ug/m poly(I:C)
Specification
mouse hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Sep 01 2015



Here are answers to your questions:

1. Since the immunogen is full length and the antibody is polyclonal this antibody will react with all forms of casepase 3.

2. The 2 bands with lower molecular weights below 22kDa are p17 ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle (AT))
Loading amount
50 µg
Specification
Skeletal muscle (AT)
Gel Running Conditions
Reduced Denaturing (15% gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

提交于 Sep 28 2012

Thank you for your reply.

There are a few things I would suggest may help improve the results:

1. I would suggest loading a higher quantity onto your gel, a good starting point would be 20 ug/lane.

2. I would suggest not u...

Read More

Thank you for contacting us.

I do not currenlty have acess to the dilution of secondary antibody used for theWestern blot presented on the datasheet of the Anti-Caspase 3 antibody (ab90437). However, we have a further report of the anitbody u...

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Thank you for completing our survey of your experience with Abcam's Scientific support.

I just wanted to follow up since your replies indicate that the issues you were having with the caspsase 3 antibody, ab90437, were not resolved satisfact...

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