Caspase 3 (active) Red Staining试剂盒(ab65617)

概述

  • 产品名称
    Caspase 3 (active) Red Staining试剂盒
    参阅全部 Caspase 3 试剂盒
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型
    Enzyme activity
  • 检测时间
    2h 00m
  • 产品概述

    Abcam's Caspase 3 (active) Red Staining Kit provides a convenient means for detecting activated caspase 3 in living cells. The assay utilizes the caspase 3 inhibitor DEVD-FMK conjugated to sulfo-rhodamine (Red-DEVD-FMK) as the fluorescent in situ marker. Red-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells.
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  • 说明

    Activation of caspases plays a central role in apoptosis.

  • 经测试应用
    适用于: FM, Functional Studies, Flow Cytmore details

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 100 tests
    Red-DEVD-FMK 1 x 100µl
    Wash Buffer 2 x 100ml
    Z-VAD-FMK 1 x 10µl
  • 研究领域
  • 相关性
    The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 (also known as CPP32, YAMA and apopain) is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as an inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kD) and small (12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 - Ser29 and Asp175 - Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (e.g. mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
  • 细胞定位
    Cytoplasmic
  • 别名
    • CASP3
    • CPP32
    • LICE
    • SCA1
    • SREBP cleavage activity 1
    • Yama protein
    see all

应用

Our Abpromise guarantee covers the use of ab65617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
FM Use at an assay dependent concentration.
Functional Studies Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.

图片

  • Caspase 3 activity in Jurkat cells (3 x10e5 cells) following 24 hour exposure to 2 µM Camptothecin (ab120115) with or without 50 μM caspase inhibitor Z-VAD(OMe)-FMK (ab120487). Background signal subtracted, duplicates; +/- SD.

实验方案

文献

ab65617 has not yet been referenced specifically in any publications.

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