Caspase-2抑制剂Assay试剂盒(ab102490)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Adherent cells, Suspension cells
概述
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产品名称
Caspase-2抑制剂Assay试剂盒
参阅全部 Caspase-2 试剂盒 -
检测方法
Fluorescent -
样品类型
Adherent cells, Suspension cells -
检测类型
Quantitative -
检测时间
1h 30m -
产品概述
Abcam's Caspase 2 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate VDVAD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 2 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 2 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
Visit our FAQs page for tips and troubleshooting. -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K152 Caspase-2 Inhibitor Drug Screening Kit (Fluorometric). K152-100 is the same size as the 100 test size of ab102490.
Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests 2X Reaction Buffer 1 x 10ml Active Caspase-2 1 x 100 units Caspase Inhibitor, Z-VAD-FMK 1 x 10µl Caspase Substrate VDVAD-AFC 1 x 0.5ml DTT 1 x 100µl -
研究领域
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功能
Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. -
组织特异性
Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle. -
序列相似性
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
翻译后修饰
The mature protease can process its own propeptide, but not that of other caspases. - Information by UniProt
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别名
- Casp 2L Pro
- CASP-2
- Casp2
see all
相关产品
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Related Products
图片
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Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using VDVAD-AFC as caspase 2 substrate and following Caspase 2 Inhibitor Drug Detection Kit (ab102490) protocol. Lanes:
1.- Background Control: no Caspase 2, no z-VMAD-FMK
2.- Positive Inhibition Control: no Caspase 2, +z-VAD-FMK
3.- Positive Control: + Caspase 2, no z-VAD-FMK
4.- Caspase 2 + 2.5µM z-VAD-FMK
5.- Caspase 2 + 10µM z-VAD-FMK
6.- Caspase 2 + 40µM z-VAD-FMK
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab102490 尚未被引用在任何文献中。