Caspase 2 Assay试剂盒(Fluorometric) (ab39794)

概述

  • 产品名称
    Caspase 2 Assay试剂盒(Fluorometric)
    参阅全部 Caspase 2 试剂盒
  • 样品类型
    Tissue Extracts, Cell Lysate
  • 检测类型
    Enzyme activity
  • 检测时间
    2h 00m
  • 产品概述

    Abcam's Caspase 2 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on detection of cleavage of substrate VDVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase 2 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader.
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  • 说明

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • 经测试应用
    适用于: Functional Studiesmore details

性能

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应用

Our Abpromise guarantee covers the use of ab39794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

实验方案

文献

ab39794 has not yet been referenced specifically in any publications.

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