Caspase 2 Assay试剂盒(Colorimetric) (ab39830)

概述

  • 产品名称Caspase 2 Assay试剂盒(Colorimetric)
    参阅全部 Caspase 2 试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Tissue Extracts, Cell Lysate
  • 检测类型Enzyme activity
  • 检测时间
    2h 00m
  • 产品概述

    Abcam's Caspase 2 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate VDVAD-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 功能Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
  • 组织特异性Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
  • 序列相似性Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 翻译后修饰The mature protease can process its own propeptide, but not that of other caspases.
  • Information by UniProt
  • 别名
    • CASP-2
    • Casp2
    • CASP2_HUMAN
    • Caspase-2 subunit p12
    • Caspase2
    • ICH 1L
    • ICH1
    • ICH1 protease
    • ICH1L
    • ICH1L/1S
    • NEDD-2
    • NEDD2
    • Neural precursor cell expressed developmentally down-regulated protein 2
    • Protease ICH-1
    see all

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应用

Our Abpromise guarantee covers the use of ab39830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

实验方案

Caspase 2 Assay Kit (Colorimetric) (ab39830)参考文献

ab39830 has not yet been referenced specifically in any publications.

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