概述

  • 产品名称Anti-Caspase-2抗体[Y154]
    参阅全部 Caspase-2 一抗
  • 描述
    兔单克隆抗体[Y154] to Caspase-2
  • 特异性This antibody is specific for the pro form and the p18 cleaved form of Caspase-2.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caspase-2 aa 250-350 (C terminal).
    Database link: P42575

  • 阳性对照
    • Jurkat cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab32021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).

Detects bands of approximately 50 kDa (pro form) and 18 kDa (p18 cleaved form).

靶标

  • 功能Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
  • 组织特异性Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
  • 序列相似性Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 翻译后修饰The mature protease can process its own propeptide, but not that of other caspases.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CASP 2 antibody
    • CASP-2 antibody
    • Casp2 antibody
    • CASP2_HUMAN antibody
    • Caspase 2 antibody
    • Caspase 2 apoptosis related cysteine peptidase antibody
    • Caspase-2 subunit p12 antibody
    • Caspase2 antibody
    • ICH 1 antibody
    • ICH 1 protease antibody
    • ICH 1L antibody
    • ICH1 antibody
    • ICH1 protease antibody
    • ICH1L antibody
    • NEDD-2 antibody
    • NEDD2 antibody
    • Neural precursor cell expressed developmentally down-regulated protein 2 antibody
    • PPP1R57 antibody
    • Protease ICH-1 antibody
    • Protein phosphatase 1 regulatory subunit 57 antibody
    see all

Anti-Caspase-2 antibody [Y154] 图像



  • Predicted band size : 49 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Caspase 2 knockout HAP1 cell lysate (20 µg)

    Lanes 1 and 2: Merged signal (red and green). Green - ab182738 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.
    ab32021 was shown to recognize caspase 2 when caspase 2 knockout samples were used, along with additional cross-reactive bands. Wild-type and caspase 2 knockout samples were subjected to SDS-PAGE. ab32021 and ab8226 (loading control to beta actin) were diluted 1/50 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Anti-Caspase-2 antibody [Y154] (ab32021) at 1/500 dilution + Jurkat cell lysate.

    Predicted band size : 49 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 18 kDa (possible cleavage fragment).

Anti-Caspase-2 antibody [Y154] (ab32021)参考文献

This product has been referenced in:
  • Shen Q  et al. Regulation of CRADD-caspase 2 cascade by histone deacetylase 1 in gastric cancer. Am J Transl Res 6:538-47 (2014). Read more (PubMed: 25360218) »
  • Yoon JY  et al. Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B. Blood Cancer J 3:e153 (2013). Human, Human, Human, Human . Read more (PubMed: 24141622) »

See all 6 Publications for this product

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