重组Anti-Caspase-2抗体[Y154] (ab32021)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y154] to Caspase-2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-Caspase-2抗体[Y154]
参阅全部 Caspase-2 一抗 -
描述
兔单克隆抗体[Y154] to Caspase-2 -
宿主
Rabbit -
特异性
This antibody is specific for the pro form and the p18 cleaved form of Caspase-2. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Caspase-2 aa 250-350 (C terminal). The exact sequence is proprietary.
Database link: P42575 -
阳性对照
- Jurkat cell lysate.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y154 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32021于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
Detects bands of approximately 50 kDa (pro form) and 18 kDa (p18 cleaved form). |
说明 |
---|
WB
1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa). Detects bands of approximately 50 kDa (pro form) and 18 kDa (p18 cleaved form). |
靶标
-
功能
Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. -
组织特异性
Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle. -
序列相似性
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
翻译后修饰
The mature protease can process its own propeptide, but not that of other caspases. - Information by UniProt
-
数据库链接
- Entrez Gene: 835 Human
- Omim: 600639 Human
- SwissProt: P42575 Human
- Unigene: 368982 Human
-
别名
- CASP 2 antibody
- CASP-2 antibody
- Casp2 antibody
see all
图片
-
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Caspase 2 knockout HAP1 cell lysate (20 µg)Lanes 1 and 2: Merged signal (red and green). Green - ab182738 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.
ab32021 was shown to recognize caspase 2 when caspase 2 knockout samples were used, along with additional cross-reactive bands. Wild-type and caspase 2 knockout samples were subjected to SDS-PAGE. ab32021 and ab8226 (loading control to beta actin) were diluted 1/50 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
Anti-Caspase-2 antibody [Y154] (ab32021) at 1/500 dilution + Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa (possible cleavage fragment)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (8)
ab32021 被引用在 8 文献中.
- Mizoguchi A et al. MicroRNA-8073: Tumor suppressor and potential therapeutic treatment. PLoS One 13:e0209750 (2018). PubMed: 30589909
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
- Majumder P et al. Cellular levels of Grb2 and cytoskeleton stability are correlated in a neurodegenerative scenario. Dis Model Mech 10:655-669 (2017). PubMed: 28360125
- Yang GW et al. Ferulic Acid Exerts Anti-Angiogenic and Anti-Tumor Activity by Targeting Fibroblast Growth Factor Receptor 1-Mediated Angiogenesis. Int J Mol Sci 16:24011-31 (2015). PubMed: 26473837
- Shen Q et al. Regulation of CRADD-caspase 2 cascade by histone deacetylase 1 in gastric cancer. Am J Transl Res 6:538-47 (2014). PubMed: 25360218
- Jelínek M et al. Caspase-2 is involved in cell death induction by taxanes in breast cancer cells. Cancer Cell Int 13:42 (2013). WB . PubMed: 23672670
- Yoon JY et al. Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B. Blood Cancer J 3:e153 (2013). Human, Human, Human, Human . PubMed: 24141622
- Aiyar SE et al. TMS, a chemically modified herbal derivative of resveratrol, induces cell death by targeting Bax. Breast Cancer Res Treat 124:265-77 (2010). PubMed: 20490654