Caspase 2 (active) FITC Staining试剂盒(ab65612)

概述

  • 产品名称
    Caspase 2 (active) FITC Staining试剂盒
    参阅全部 Caspase 2 试剂盒
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型
    Enzyme activity
  • 检测时间
    2h 00m
  • 产品概述

    Abcam's Caspase 2 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase 2 in living cells. The assay utilizes the caspase 2 inhibitor, VDVAD-FMK, conjugated to FITC (FITC-VDVAD-FMK) as a marker. FITC-VDVAD-FMK is cell permeable, non-toxic, and irreversibly binds to activated caspase 2 in apoptotic cells. The FITC label allows detection of activated caspase 2 in apoptotic cells directly by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Activation of caspases plays a central role in apoptosis.

  • 经测试应用
    适用于: FM, Functional Studies, Flow Cytmore details

性能

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应用

Our Abpromise guarantee covers the use of ab65612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
FM Use at an assay dependent concentration.
Functional Studies Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.

实验方案

文献

ab65612 has not yet been referenced specifically in any publications.

客户评价及客户问答

The developer of the kit suggests trypsinizing the cells and then using the protocol for cells in suspension. If you do the assay this way, it would probably be best any apply the apoptosis-inducing treatment to the cells after trypsinization, to the c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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