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Abcam's Caspase 2 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase 2 in living cells. The assay utilizes the caspase 2 inhibitor, VDVAD-FMK, conjugated to FITC (FITC-VDVAD-FMK) as a marker. FITC-VDVAD-FMK is cell permeable, non-toxic, and irreversibly binds to activated caspase 2 in apoptotic cells. The FITC label allows detection of activated caspase 2 in apoptotic cells directly by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
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Activation of caspases plays a central role in apoptosis.
|FITC-VDVAD-FMK||1 x 100µl|
|Wash Buffer||2 x 100ml|
|Z-VAD-FMK||1 x 10µl|
Our Abpromise guarantee covers the use of ab65612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|FM||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent dilution.|
|Flow Cyt||Use at an assay dependent dilution.|
ab65612 has not yet been referenced specifically in any publications.