Caspase 2 (active) FITC Staining试剂盒(ab65612)

概述

  • 产品名称Caspase 2 (active) FITC Staining试剂盒
    参阅全部 Caspase 2 试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Enzyme activity
  • 检测时间
    2h 00m
  • 产品概述

    Abcam's Caspase 2 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase 2 in living cells. The assay utilizes the caspase 2 inhibitor, VDVAD-FMK, conjugated to FITC (FITC-VDVAD-FMK) as a marker. FITC-VDVAD-FMK is cell permeable, non-toxic, and irreversibly binds to activated caspase 2 in apoptotic cells. The FITC label allows detection of activated caspase 2 in apoptotic cells directly by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Activation of caspases plays a central role in apoptosis.

  • 经测试应用适用于: FM, Functional Studies, Flow Cytmore details

性能

  • 功能Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
  • 组织特异性Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
  • 序列相似性Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 翻译后修饰The mature protease can process its own propeptide, but not that of other caspases.
  • Information by UniProt
  • 别名
    • CASP-2
    • Casp2
    • CASP2_HUMAN
    • Caspase-2 subunit p12
    • Caspase2
    • ICH 1L
    • ICH1
    • ICH1 protease
    • ICH1L
    • ICH1L/1S
    • NEDD-2
    • NEDD2
    • Neural precursor cell expressed developmentally down-regulated protein 2
    • Protease ICH-1
    see all

相关产品

应用

Our Abpromise guarantee covers the use of ab65612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
FM Use at an assay dependent concentration.
Functional Studies Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.

实验方案

Caspase 2 (active) FITC Staining Kit (ab65612)参考文献

ab65612 has not yet been referenced specifically in any publications.

Product Wall

The developer of the kit suggests trypsinizing the cells and then using the protocol for cells in suspension. If you do the assay this way, it would probably be best any apply the apoptosis-inducing treatment to the cells after trypsinization, to the c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"