概述

  • 产品名称Anti-Caspase-14抗体
    参阅全部 Caspase-14 一抗
  • 描述
    兔多克隆抗体to Caspase-14
  • 特异性This antibody is specific for Caspase-14.
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-14 (C terminal).
    Database link: P31944
    (Peptide available as ab72738)

  • 阳性对照
    • Jurkat cell lysate This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2 and HeLa.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Caspase-14 Antibody is affinity chromatography purified via peptide column.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab45415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2.5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).Can be blocked with Caspase-14 peptide (ab72738).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Believed to be a non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and cornification. Probably regulates maturation of the epidermis by proteolytically processing filaggrin.
  • 组织特异性Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels.
  • 序列相似性Belongs to the peptidase C14A family.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apoptosis related cysteine protease antibody
    • CASP 14 antibody
    • CASP-14 antibody
    • CASP14 antibody
    • Caspase 14 apoptosis related cysteine protease antibody
    • Caspase 14 precursor antibody
    • Caspase-14 subunit p10 antibody
    • Caspase14 antibody
    • CASPE_HUMAN antibody
    • MGC119078 antibody
    • MGC119079 antibody
    • MICE antibody
    • Mini ICE antibody
    see all

Anti-Caspase-14 antibody 图像

  • ab45415 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab45415 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5min) HeLa cells at 5ug/ml.

  • All lanes : Anti-Caspase-14 antibody (ab45415) at 1 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate + blocking peptide

    Secondary
    anti-rabbit IgG

    Predicted band size : 27 kDa
    Observed band size : 27 kDa

Anti-Caspase-14 antibody (ab45415)参考文献

This product has been referenced in:
  • Asselin-Labat ML  et al. Gata-3 negatively regulates the tumor-initiating capacity of mammary luminal progenitor cells and targets the putative tumor suppressor caspase-14. Mol Cell Biol 31:4609-22 (2011). WB . Read more (PubMed: 21930782) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Keratinocytes)
Loading amount 20 µg
Specification Keratinocytes
Gel Running Conditions Reduced Denaturing (4~12% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. HYOUNG JUNE KIM

Verified customer

提交于 Oct 26 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"