概述

  • 产品名称
    Caspase 12 Assay试剂盒(Fluorometric)
    参阅全部 Caspase 12 试剂盒
  • 样品类型
    Tissue Extracts, Cell Lysate
  • 检测类型
    Enzyme activity
  • 检测时间
    2h 00m
  • 产品概述

    Abcam's Caspase 12 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence ATAD. The assay is based on detection of cleavage of substrate ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). ATAD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase 12 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • 经测试应用
    适用于: Functional Studiesmore details

性能

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应用

Our Abpromise guarantee covers the use of ab65664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

实验方案

文献

This product has been referenced in:
  • Bulatovic I  et al. Sublethal caspase activation promotes generation of cardiomyocytes from embryonic stem cells. PLoS One 10:e0120176 (2015). Functional Studies . Read more (PubMed: 25763592) »
  • Mitra A  et al. Cardioprotective role of P38 MAPK during myocardial infarction via parallel activation of a-crystallin B and Nrf2. J Cell Physiol 229:1272-82 (2014). Functional Studies . Read more (PubMed: 24464634) »

See all 2 Publications for this product

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