Anti-Caspase-12抗体(ab87348)

概述

  • 产品名称Anti-Caspase-12抗体
    参阅全部 Caspase-12 一抗
  • 描述
    兔多克隆抗体to Caspase-12
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Cow, Dog, Human, Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to Mouse Caspase-12 aa 400 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q6UXS9
    (Peptide available as ab98317, ab98329, ab98329)

  • 阳性对照
    • This antibody gave a positive signal in Mouse and Rat Spleen tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab87348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).

靶标

Anti-Caspase-12 antibody 图像

  • All lanes : Anti-Caspase-12 antibody (ab87348) at 1 µg/ml

    Lane 1 : Spleen (Rat) Tissue Lysate
    Lane 2 : Spleen (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

Anti-Caspase-12 antibody (ab87348)参考文献

ab87348 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Dog Cell lysate - whole cell (MDCK)
Specification MDCK
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 28 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (rat podocyte)
Loading amount 30 µg
Specification rat podocyte
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

提交于 Apr 11 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"