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Synthetic peptide corresponding to Mouse Caspase-12. immunogen sequence from within aa 181 to 240
Database link: O08736
Our Abpromise guarantee covers the use of ab62484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38-45 kDa (predicted molecular weight: 38-45 kDa). This antibody will detect full length Casp12 (42 kDa) and also the cleaved active form (38 kDa) in the appropriate lysates.|
|IHC-P||Use a concentration of 2 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 18621034|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
Paraffin-embedded rat heart tissue stained for Caspase-12 using ab62484 at 2.5 µg/ml dilution in immunohistochemical analysis.
Immunohistochemical analysis of human heart tissue labelling Caspase-12 with ab62484 at 10 µg/mL.
This image is courtesy of an abreview submitted by Karine Thibault, CNRS, Paris, FranceThe full length Casp12 (42 kDa) observed in control rat was cleaved into an active form with a size of 38 kDa in treated rat
Paraffin-embedded human heart tissue stained for Caspase-12 using ab62484 at 2 µg/ml dilution in immunohistochemical analysis.
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